Quantification of an antibody

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ELISA and Hybridoma

Antibody – antigen interactions might involve all types of chemical bonding, except which one? Explain.

07 August 2017 2,293 5 View

Western Blotting

Why must 1. electrophoresis of the protein sample, and 2. transfer to nitrocellulose precede the actual probing of the sample with an antibody?

07 August 2017 9,508 1 View

All immunogens are antigens, but not all antigens are imunogens.?

Explain the statement “All immunogens are antigens, but not all antigens are imunogens.”

07 August 2017 4,712 7 View

ELISA and Immunohistochemistry

Why Immunohistochemistry requires a conjugated enzyme, whereas immunofluorescence does not. Explain the difference?

07 August 2017 945 5 View

ELISA and Hybridoma Technology

An investigator wanted to produce rabbit antiserum specific for mouse IgG, so she injected a rabbit with purified mouse IgG. However, to her dismay, the antiserum reacted with all isotypes of...

07 August 2017 8,415 7 View

ELISA

I am planning to run an ELISA soon but I have a question regarding sample preparation. If I don’t know the working range of my samples for the ELISA, should I just add the samples to the plate...

07 August 2017 5,462 4 View

ELISA, Immunoprecipitation and Affinity Chromatography

What are some common treatments that could be used to disrupt the antibody-antigen complexes?

07 August 2017 5,565 4 View

Western Blotting and ELISA

What is the function of the enzyme bound to the secondary antibody?

07 August 2017 8,198 9 View

Dilution of Samples in ELISA

What is the need for serial dilution of the sample when performing an Elisa?

07 August 2017 1,692 1 View

Western Blot, ELISA and Immunohistochemistry

What are the differences and similarities between western blotting and immunohistology?

07 August 2017 2,178 3 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

Weam Al-Hamadany

Is it specific or non- specific Ab?

Mayank Garg

Enzyme-linked immunosorbent assay (ELISA) based methods are the most efficient in quantifying the level of antibody in a given sample.

Gertrudis Rojas

Which kind of sample? Polyclonal or monoclonal antibodies? Do you want to measure specific antibodies or total antibody content? The ELISA design depends on those answers.