Can anyone suggest normalizing genes for real time PCR (qPCR) if I my sample is DNA from (mouse) McCoy fibroblast cells?
Some of the popular ones are 18S, Beta-actin, and Beta-2 microglobulin.
G6PDH can also be used.
Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...
07 February 2021 355 2 View
I need to know how to plot the impact of pressure (0 - 100 kPa) (which is applied on the back side of diaphragm) on the frequency shift caused in the cc beam supported on the diaphragm with...
01 February 2021 5,805 17 View
I have developed a project based on Arduino that communicates through Bluetooth with Android application, I want to enhance its privacy through encryption so that data should be prevented up to...
07 January 2021 7,933 5 View
While working on IoT projects I mostly use NodeMCU ESP8266 firmware for IoT Implementation. For coding, I use Arduino IDE in which for connectivity with wifi router we define hardcoded variable...
07 January 2021 6,688 3 View
What's the reasonable loading control of nuclear protein in acute injury? Is HDAC1 or Histone H3 a resonable loading control of nuclear protein in acute injury model? How to explain total my total...
06 January 2021 1,607 2 View
The SARS-CoV-2 virus spreading across the world has led to surges of COVID-19 illness, hospitalizations, and death. The complex and multifaceted pathophysiology of life-threatening COVID-19...
29 December 2020 7,446 4 View
I am looking for a document that summarises mathematical results for optimisation techniques : steepest descent, newton and quasi-Newton, their convergence speed in different settings...
19 December 2020 2,908 8 View
I'm looking for a company to sequence the complete mitochondrial genome of some species of fish.
14 December 2020 3,154 3 View
I got these parasites from eye and intestine of a marine fish. Thank you.
09 December 2020 7,752 2 View
Hello You can see a transversal slice of a mouse heart in microscopie with blue coloration. My question : what part of the heart is it ? how i can recognize difference between histology of...
01 December 2020 1,000 3 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
02 March 2021 3,060 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View