Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

How to design manholes of a stormwater tunnel?

I am exploring for the design code or manual and standard drawings of access and maintenance manholes of the storm water conveying tunnels. (i.e free flow tunnels not pressurized ones) but all of...

22 December 2020 2,590 4 View

How to use TransDecoder for transcriptome analysis?

I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...

01 December 2020 4,632 3 View

Offshore steel structure of wind turbines 3D samples ?

i want to do a study on offshore wind turbines on sap model , initial study for graduation project of steel diploma (at ainshams university) , kindly if any specialized in this field can help me...

05 November 2020 3,571 4 View

SAS code for a "Split-plot in time" (based on CRD design)?

Dear all, I want to analyze a factorial split-plot in time using SAS. Factorial Experiment using Completely Randomized Design (CRD); Factor A: treatments (a1-a4) Factor B: harvest time,...

28 October 2020 3,515 4 View

Is there a solvent for blending PHB and Acid Ascorbic (via solution casting)?

Dear Researchers I want to add Ascorbic Acid (AA) to PHB in order to improve its biodegradability. AA is soluble in water but insoluble in those that are compatible with PHB (THF, Chloroform,...

07 October 2020 1,136 4 View

Journal vs conference publications, which one would you prefer ?

For sure journal publications have more weight than conference publications but i am asking about your preference. For example i like conferences a lot because i like to meet people and explore...

06 September 2020 7,468 42 View

What is best software for simulation of microfluidic?

I'm working on a microfluidic project. I need a software to simulate all forces include: centrifugal (surely Euler and Coriolis too), and surface tension. Solidworks follow simulation doesn't...

30 August 2020 4,671 9 View

Do ammonium glutamate considered as necessity in salt sloution for Ecoli-based Cell free protein synthesis platform ?

Hello, I am constructing an E-coli based Cell free protein system (CFPS) reagent, but I can't find ammonium glutamate from the local suppliers or from MP biochemical local disruptors. Can I...

13 August 2020 3,691 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

Who are the possible experts I can reach out to regarding the reseach topic: Activated Carbon as Water Filter?

Hi! Me and my team mates are currently new in doing a research study. We do not have any previous experiences in research. And we just wanted to know what type of experts (the field they in) can...

19 February 2021 9,596 4 View

Comsol Maximum number of Newton iterations reached?

Comsol is investigating deformation due to gravity. Gravity is applied to the nanosheets to check for displacement and stress. If I input the Young's modulus of the channel to about 1 [GPa], the...

15 February 2021 6,027 3 View

What parameters of anodizing I should use?

19 January 2021 3,792 3 View

Can anyone help for the content validity of my psychological test?

Good day! I am in the midst of completing my test development requirement under the Psychological Assessment subject. I was wondering if anyone can help me reach experts or psychology graduates...

14 January 2021 1,536 4 View

Marker Set for 3D Motion Tracking of Upper Arm during Pointing Task?

Does anyone have recommendations of a marker set for 3D motion tracking of the upper arm during a pointing task? Most studies I have come across have focused on reaching. I plan to use Vicon Nexus...

12 January 2021 3,198 1 View

Do drugs that are injected i.t in rats reach the supraspinal system and how long does it take to clear them from spinal cord?

I am injecting intrathecally (between L5-L6) , in rats, cannabinoid analgesics and I was wondering if they can reach the brain and have effects there as well ? Also I need to pretreat with...

07 January 2021 5,397 4 View

Homeopathy is within the reach of most of the financial class?

Greetings. I personally have taken homeopathy medications and found it effective in curing. I do not know if it is effective in all kinds of diseases, but it is within the financial reach of all...

02 January 2021 9,904 5 View

No title

What are the technicalities involved ? What are the reasons ? Can we share some facts on this

31 December 2020 5,698 5 View