Global management market of courses & marketing to improve quality in Pt Treatment or science or mainly Bureaucracy?

12 October 2020 9,272 1 View

SEM: What does CFI = 1 & RMSEA = 0 indicate in over-identified models?

I'm suspicious about "perfect fit" in my SEM path analysis, with fit indices of CFI = 1 and RMSEA = 0 which I would expect from an just-identified model. However, since I have 3 degrees of...

08 August 2020 1,492 4 View

Data on most frequently researched KSAOs or personality traits?

I'm looking for meta analyses or reviews, summarizing which knowledge, skills, abilities and other characteristics (KSAOs, especially personality traits) are most frequently researched in...

22 May 2020 332 1 View

AC/DC inverters: Are there any standards or laws regulation DC output on the AC Grid?

Hi, we are working on internal specifications on On-Board Charger for Battery electric vehicles. We have seen some field failures on older generations. We are suspecting that e.g. PV AC/DC...

15 December 2019 6,887 1 View

How to test unidimensionality for polytomous data with IRT-models in R?

I would be thankful if someone could give me a short breakdown on how to test unidimensionality of polytomous data (likert-scale) using IRT-models in R. As it is my understanding that the...

22 November 2019 6,730 7 View

How do you optimize your research design for questionnaires with large item pools?

While developing a questionnaire to measure several personality traits in a somewhat unconventional way, I now seem to be facing a dilemma due to the size of my item pool. The questionnaire...

30 May 2019 2,633 4 View

What factors generally improve inter-rater reliability?

I'm developing a questionnaire to assess observational data in an area of research which is notoriously prone to low inter-rater reliability. Hence, I'm looking for factors in questionnaire design...

03 December 2018 4,006 3 View

Machine learning in economic research?

What is your take on machine learning as an applied method in economic research. is it a game changer or will it not impact your approach to research?

22 July 2018 7,401 5 View

Is there any way to statistically test control samples with parent material?

I want to statistically test the relative gene expression of precision cut liver slices with the gene expression of their parent livers. I have 14 samples from 3 different parent livers that I...

09 March 2018 4,517 4 View

What is the average magnitude of correlations between common personnel selection methods?

Looking for recent (preferably meta-analytic) findings that yielded estimates of the proportion of shared variance among common personnel selection methods such as structured and unstructured...

11 December 2017 1,946 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to annotate list of gene names using Bash/R?

I have a list of gene names from deep sequencing from a constructed CRISPR knockout library. I was hoping to write a script to annotate all the gene names with a quick description of product...

27 January 2021 8,035 1 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Can anyone explain why my starters are amplifying sequence from human genome instead of HPV16?

Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....

24 January 2021 7,636 3 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View