I am searching for a protocol to fuse Arabidopsis protoplasts. Could you help me?
Thanks in advance
Dear colleagues, We are starting NBRIP assays in the lab to evaluate the capacity of rhizobacterial strains of our interest to solubilize calcium phosphate. To our surprise, the yellow/destained...
21 December 2021 1,309 0 View
I am new in the field of plant nutrition and I am searching for the most relevant markers to use to follow N and P starvation in Arabidopsis (both in shoots and roots). What are the most sensitive...
08 November 2021 8,135 3 View
Hi everyone, I received a piece of operative tissue in a frozen "cryomold". Im a not familiar with this thing. I wanted to do RNA extraction from this tissue. I don't know how to recover the...
08 August 2021 2,584 0 View
Dear peers, I need some advice to revive strains of Hyphomicrobium that have been stored at -80°C as glycerol stocks (as part of a reference stock). I have tried to culture them again on plates...
11 February 2021 3,753 2 View
Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...
11 November 2020 835 3 View
Hi, I'm working on exosomes isolated from cell supernatant of cultured cells. Briefly, I accumulate 100 - 200 ml of supernatant and process it until a concentration in a final volume of 50µL....
20 July 2020 1,905 7 View
I set the threshold of my reactions in the linear graph view, at the beginning of the exponential phase of the amplification. In the logarithmic graph the threshold is set at the middle of the...
23 June 2020 2,483 0 View
To know if you plan to add videos from other laboratories, so that the channel has several types of techniques - like the operation of SEM, XRD, GIXRD, AFM ... Good idea anyway!
09 April 2020 3,946 0 View
Hi everyone, I would like to fragment DNA of dolphins using qSonica to obtain fragment size between 300-350bp in order to prepare a library for Whole-Genome-Sequencing. I have already run...
26 February 2020 5,197 0 View
Hello, I'm trying to capture viral RNA from my total RNA fraction so i've extracted RNA with trizol and digest with DNase. Then I've design 7 RNA-probes of 40 nucleotide each to span all the...
24 February 2020 7,171 2 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View