07 July 2013 1 9K Report

I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!

More Alejandro Gomez Mejia's questions See All
Any tips for good mRNA extraction from bacteria?

I would like to know which are the most important points I should consider when performing mRNA extraction from bacteria.

31 December 2014 4,546 4 View

How accurate are the pre stained protein MW markers for Western blot?

I'm currently doing a Western blot for a protein with a MW of 52 kDa, but when I do my Western blot, the band shows up somewhere below the 70 Kda, but higher than 55 kDa. So it makes me worry a...

04 May 2014 8,202 3 View

What is the minimum amount of protein you should load in an SDS-PAGE for detection with coomassie blue staining R-250?

I've been trying to extract proteins from microalgae, and I'm getting around 0.7 mg/mL from a 20 mg pellet, but when I load it on my SDS-PAGE I'm not getting any bands, according to my calculation...

01 February 2014 2,631 11 View

Western blot for microalgal protein extraction?

I would like to learn about methodologies for protein extraction from microalgae (Scenedesmus and Chlorella) using the smallest amount of biomass as possible for a western blot assay.

31 December 2013 9,362 6 View

Tips on fragment ligation?

I want to insert a PCR amplified fragment into a vector, the thing is I have to use only one enzyme for the digestion, and I'm getting no colonies at all, dephosphorylation is with Alkalyne...

09 October 2013 2,755 19 View

Hygromycin B resistance in microalgae?

I'm working with Scenedesmus obliquus and Chlorella vulgaris and I'm using hygromycin as a selection antibiotic for a genetic modification. Can you help me on how much hygromycin should I use for...

09 October 2013 5,828 2 View

Microalgae DNA extraction?

I've been trying to isolate DNA from Chlorella, Scenedesmus vacuolatus and Scenedesmus obliquus but I have been unsuccessful. I have been using dry ice and CTAB buffer, but I get really low...

07 August 2013 463 15 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close