I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!
Fisher Per Plant kit works well for proteins.
I would like to know which are the most important points I should consider when performing mRNA extraction from bacteria.
31 December 2014 4,461 4 View
I'm currently doing a Western blot for a protein with a MW of 52 kDa, but when I do my Western blot, the band shows up somewhere below the 70 Kda, but higher than 55 kDa. So it makes me worry a...
04 May 2014 8,119 3 View
I've been trying to extract proteins from microalgae, and I'm getting around 0.7 mg/mL from a 20 mg pellet, but when I load it on my SDS-PAGE I'm not getting any bands, according to my calculation...
01 February 2014 2,555 11 View
I would like to learn about methodologies for protein extraction from microalgae (Scenedesmus and Chlorella) using the smallest amount of biomass as possible for a western blot assay.
31 December 2013 9,282 6 View
I'm working with Scenedesmus obliquus and Chlorella vulgaris and I'm using hygromycin as a selection antibiotic for a genetic modification. Can you help me on how much hygromycin should I use for...
09 October 2013 5,762 2 View
I want to insert a PCR amplified fragment into a vector, the thing is I have to use only one enzyme for the digestion, and I'm getting no colonies at all, dephosphorylation is with Alkalyne...
09 October 2013 2,649 19 View
I've been trying to isolate DNA from Chlorella, Scenedesmus vacuolatus and Scenedesmus obliquus but I have been unsuccessful. I have been using dry ice and CTAB buffer, but I get really low...
07 August 2013 375 15 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View