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Questions related from Alejandro Gomez Mejia
I would like to know which are the most important points I should consider when performing mRNA extraction from bacteria.
01 January 2015 4,507 4 View
I'm currently doing a Western blot for a protein with a MW of 52 kDa, but when I do my Western blot, the band shows up somewhere below the 70 Kda, but higher than 55 kDa. So it makes me worry a...
05 May 2014 8,166 3 View
I've been trying to extract proteins from microalgae, and I'm getting around 0.7 mg/mL from a 20 mg pellet, but when I load it on my SDS-PAGE I'm not getting any bands, according to my calculation...
02 February 2014 2,600 11 View
I would like to learn about methodologies for protein extraction from microalgae (Scenedesmus and Chlorella) using the smallest amount of biomass as possible for a western blot assay.
01 January 2014 9,328 6 View
I want to insert a PCR amplified fragment into a vector, the thing is I have to use only one enzyme for the digestion, and I'm getting no colonies at all, dephosphorylation is with Alkalyne...
10 October 2013 2,696 19 View
I'm working with Scenedesmus obliquus and Chlorella vulgaris and I'm using hygromycin as a selection antibiotic for a genetic modification. Can you help me on how much hygromycin should I use for...
10 October 2013 5,796 2 View
I've been trying to isolate DNA from Chlorella, Scenedesmus vacuolatus and Scenedesmus obliquus but I have been unsuccessful. I have been using dry ice and CTAB buffer, but I get really low...
08 August 2013 425 15 View
I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!
07 July 2013 8,981 1 View
