How accurate are the pre stained protein MW markers for Western blot?

I'm currently doing a Western blot for a protein with a MW of 52 kDa, but when I do my Western blot, the band shows up somewhere below the 70 Kda, but higher than 55 kDa. So it makes me worry a...

04 May 2014 8,119 3 View

What is the minimum amount of protein you should load in an SDS-PAGE for detection with coomassie blue staining R-250?

I've been trying to extract proteins from microalgae, and I'm getting around 0.7 mg/mL from a 20 mg pellet, but when I load it on my SDS-PAGE I'm not getting any bands, according to my calculation...

01 February 2014 2,555 11 View

Western blot for microalgal protein extraction?

I would like to learn about methodologies for protein extraction from microalgae (Scenedesmus and Chlorella) using the smallest amount of biomass as possible for a western blot assay.

31 December 2013 9,282 6 View

Hygromycin B resistance in microalgae?

I'm working with Scenedesmus obliquus and Chlorella vulgaris and I'm using hygromycin as a selection antibiotic for a genetic modification. Can you help me on how much hygromycin should I use for...

09 October 2013 5,762 2 View

Tips on fragment ligation?

I want to insert a PCR amplified fragment into a vector, the thing is I have to use only one enzyme for the digestion, and I'm getting no colonies at all, dephosphorylation is with Alkalyne...

09 October 2013 2,649 19 View

Microalgae DNA extraction?

I've been trying to isolate DNA from Chlorella, Scenedesmus vacuolatus and Scenedesmus obliquus but I have been unsuccessful. I have been using dry ice and CTAB buffer, but I get really low...

07 August 2013 375 15 View

Protocols for fast DNA extraction and protein extraction from microalgae?

I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!

06 July 2013 8,945 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View