Any tips for good mRNA extraction from bacteria?

I would like to know which are the most important points I should consider when performing mRNA extraction from bacteria.

31 December 2014 4,461 4 View

How accurate are the pre stained protein MW markers for Western blot?

I'm currently doing a Western blot for a protein with a MW of 52 kDa, but when I do my Western blot, the band shows up somewhere below the 70 Kda, but higher than 55 kDa. So it makes me worry a...

04 May 2014 8,119 3 View

What is the minimum amount of protein you should load in an SDS-PAGE for detection with coomassie blue staining R-250?

I've been trying to extract proteins from microalgae, and I'm getting around 0.7 mg/mL from a 20 mg pellet, but when I load it on my SDS-PAGE I'm not getting any bands, according to my calculation...

01 February 2014 2,555 11 View

Western blot for microalgal protein extraction?

I would like to learn about methodologies for protein extraction from microalgae (Scenedesmus and Chlorella) using the smallest amount of biomass as possible for a western blot assay.

31 December 2013 9,282 6 View

Tips on fragment ligation?

I want to insert a PCR amplified fragment into a vector, the thing is I have to use only one enzyme for the digestion, and I'm getting no colonies at all, dephosphorylation is with Alkalyne...

09 October 2013 2,649 19 View

Microalgae DNA extraction?

I've been trying to isolate DNA from Chlorella, Scenedesmus vacuolatus and Scenedesmus obliquus but I have been unsuccessful. I have been using dry ice and CTAB buffer, but I get really low...

07 August 2013 375 15 View

Protocols for fast DNA extraction and protein extraction from microalgae?

I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!

06 July 2013 8,945 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View