I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!
Fisher Per Plant kit works well for proteins.
I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...
28 February 2021 3,637 2 View
Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...
05 February 2021 7,886 1 View
Hello, I am using Coulomb 3.3. When I try to use the option "Build input from CMT or focal mech. data" I have the next errors: Unrecognized function or variable 'deg2utm'. Error in distance2...
18 January 2021 4,487 4 View
I am trying to obtain a trend report on a specific issue at your instigation in recent years
28 December 2020 848 2 View
no details needed
26 December 2020 9,690 3 View
Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?
23 December 2020 5,058 7 View
I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...
21 December 2020 4,088 3 View
01 November 2020 1,234 3 View
Hi, I am preparing to do a western blot and I need to add 4X Laemli sample buffer to my samples before adding them to the gel and I had a few questions (I am using the Biorad 4X Laemli sample...
13 October 2020 9,485 1 View
In view of the potential for Covid-19 reinfection and the weak immune response and short duration in patients previously infected with Covid-19, many doubts have emerged if the virus is capable of...
03 October 2020 5,689 7 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View