Alejandro Gomez Mejia

4 Questions 9 Answers 0 Followers

Questions related from Alejandro Gomez Mejia

How accurate are the pre stained protein MW markers for Western blot?

I'm currently doing a Western blot for a protein with a MW of 52 kDa, but when I do my Western blot, the band shows up somewhere below the 70 Kda, but higher than 55 kDa. So it makes me worry a...

01 May 2014 3,748 3 View

What is the minimum amount of protein you should load in an SDS-PAGE for detection with coomassie blue staining R-250?

I've been trying to extract proteins from microalgae, and I'm getting around 0.7 mg/mL from a 20 mg pellet, but when I load it on my SDS-PAGE I'm not getting any bands, according to my calculation...

25 February 2014 7,296 10 View

Western blot for microalgal protein extraction?

I would like to learn about methodologies for protein extraction from microalgae (Scenedesmus and Chlorella) using the smallest amount of biomass as possible for a western blot assay.

13 January 2014 7,164 5 View

Protocols for fast DNA extraction and protein extraction from microalgae?

I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!

03 July 2013 3,619 1 View