7 Questions 28 Answers 0 Followers
Questions related from Alejandro Gomez Mejia
I'm working with in vitro phosphorylation and I would like to use a phosphatase inhibitor for my assay. Thank you very much!
22 March 2016 7,164 2 View
I'm currently doing a Western blot for a protein with a MW of 52 kDa, but when I do my Western blot, the band shows up somewhere below the 70 Kda, but higher than 55 kDa. So it makes me worry a...
01 May 2014 3,814 3 View
I've been trying to extract proteins from microalgae, and I'm getting around 0.7 mg/mL from a 20 mg pellet, but when I load it on my SDS-PAGE I'm not getting any bands, according to my calculation...
25 February 2014 7,389 10 View
I would like to learn about methodologies for protein extraction from microalgae (Scenedesmus and Chlorella) using the smallest amount of biomass as possible for a western blot assay.
13 January 2014 7,231 5 View
I'm working with Scenedesmus obliquus and Chlorella vulgaris and I'm using hygromycin as a selection antibiotic for a genetic modification. Can you help me on how much hygromycin should I use for...
10 October 2013 1,521 3 View
I want to insert a PCR amplified fragment into a vector, the thing is I have to use only one enzyme for the digestion, and I'm getting no colonies at all, dephosphorylation is with Alkalyne...
07 October 2013 3,107 18 View
I need to extract DNA and proteins from different microalgae strains but I don't wanna use TCA as it's hazardous for the environment. Any recommendations? Thanks in advance!
03 July 2013 3,665 1 View
