How to best engender critical thinking in K-12 education?

From my observations, there appear to be three principal avenues to engender critical thinking in education K-12: debate; mathematics, and science. For some students, all three avenues are...

04 February 2021 9,605 7 View

What is the best way of processing very large files in python?

I want to process a very large file, let's say 300 GB, with Python and I'm wondering what is the best way to do it. One limitation is that my algorithm needs the whole file in memory, so a...

15 January 2021 6,691 4 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

Mixed ANOVA independent variables understanding in SPSS?

I have an experiment where 10 groups of 3 judges are asked to evaluate a list of pictures in 2 evaluation conditions. The pictures were made in 2 between-subjects conditions (A,B) and each group...

20 December 2020 7,697 3 View

Any known "open" tools for organizing information for research?

I am doing a bit of research on different areas (AI, cognition, music psychology, aging...) and have gathered so far a huge amount of information in the form of web links, pdfs, printed books and...

15 December 2020 8,004 5 View

Calculation the Pump power needed in a pressurized flow system?

Hi all, I am design now a liquid high pressure flow system to be used up to 50 bar, and I am having some doubts concerning how I can estimate the pump power. Besides the pressure drop in the...

15 December 2020 8,411 1 View

Design of a membrane system to remove CO2 from a flue gas current?

Hi all, I am trying to design a membrane system to remove the CO2 from a flue gas current, and I am having difficulties in finding information. Does anyone have any technical information,...

15 December 2020 3,655 2 View

Will teleworking in the future be done from home or from shared spaces in a creative and dynamic environment, but not more than 15 minutes from home?

Teleworking works best in terms of productivity and innovation if carried out in co-working, as the critical peer review allows to continuously improve the "deliverables"

03 December 2020 7,376 5 View

What are the best sources for education in the field of Kinesiology?

As a student, I can't find good but reliable sources for expanding knowledge on the internet, so I'm looking for something easy to understand and read, not scientific journals and papers.

26 November 2020 8,560 3 View

Could you recommend a book that has change your view of seeing kinesiology (sport science, human anatomy & physiology) in a different matter?

I'm looking for something inspirational which would show me a different side of kinesiology. Something easy to understand but also educational.

26 November 2020 4,611 10 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View