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Recently, we have been encountering challenges in our laboratory in achieving satisfactory mineralization of MC3T3-E1 cell line. We would like to determine the optimal concentration of Ascorbic...
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Hi! I am trying to polarize RAW 264.7 macrophages on the 7th pass (ATCC) with 100 ng/mL LPS (Sigma) for M1 and 40 ng/mL IL4 (Sigma) for M2. They were plated at a density of 20,000 cells per well,...
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According to Quan et al. 2020, to induce M1 and M2 RAW 264.7 cell phenotypes, 50 ng/mL lipopolysaccharide (LPS) (E. coli, serotype 055:B5) and 40 ng/mL IL-4 were added to the culture,...
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