Hi, I am new to Proteome Discoverer. I have received results for phosphopeptide comparison between two mutants and control in the form of excel sheet. It shows that there are an initial 4500 phosphopeptide reads. From those, filter can be applied to remove the ones with low PSM etc. I believe that ANOVA should be performed on that filtered list. And from that, significant changes should be identified. However, our core is first doing ANOVA on the 4500 list. Then filtering the list to retain peptides with PSM and then further filtering it to pick the significant ones. But this significance is based on ANOVA done on initial 4500 peptides and.not on filtered list. I am wondering what is the right workflow to get to significant changes.