Can Stearylamine affects the extrusion process in liposomes preparation ?
Which concentration (molar ratio) or composition (lipids: Cholesterol: Stearylamine) is suitable for the encapsulation of proteins (High loading) ?
If your hydration media is slightly acidic (pH=6) you can increase zeta potential as well.
Hi,
Stearylamine (SA) is an established cationic molecule employed in drug delivery systems including liposomes and nanoliposomes. Used in correct quantity to confer a zeta potential of equal or greater than +30mV, it increases the stability of formulation.
We recommend a molar ratio of 8.0: 1.0: 0.5: 0.5 (neutral / zwitterionic lipids: stearylamine: cholesterol: antioxidant) for liposome / nanoliposome preparation. To these vesicles, targeting ligands (peptides) and/or polymers (e.g. PEG) can be incorporated.
Have a look at these literature on the use of SA in drug delivery systems:
Preliminary studies of a stearylamine-based cationic liposome
www.journalofpharmaceuticalresearch.org/index.php/kpc/article/viewFile/.../67713
by: EB Onuigbo - 2011
__ __ __
Stearylamine Liposomal Delivery of Monensin in ... - NCBI - NIH
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4775978/
by V Rajendran - 2016 -
Liposomes exhibited a spherical shape, with sizes ranging from 90 to 120 nm, as measured by dynamic light scattering and high-resolution electron microscopy. ... This is the first report to demonstrate that monensin in PEGylated stearylamine (SA) liposome has therapeutic potential against malaria infections. ...
Avinash Singh Patel If your molecule to be encapsulated is anionic, stearylamine liposomes would enhance the encapsulation efficiency. I usually employ 7:2 (DSPC:SA)
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts