The following links may be useful for measurement of melanin content:
Quantitative analysis of melanin content in a three-dimensional melanoma cell culture. Scientific Reports volume 9, Article number: 780 (2019).
https://www.nature.com/articles/s41598-018-37055-y
Methodology for evaluation of melanin content and production of pigment cells in vitro. .Photochem Photobiol May-Jun 2008;84(3):645-9. Article Methodology for Evaluation of Melanin Content and Production...
For free radical scavenging assay:
Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Science and Human Wellness
Volume 4, Issue 1, March 2015, Pages 42-46. Article Evaluation of Free Radical Scavenging Activity of Various Le...
Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Future Sci OA. 2018 Feb; 4(2): FSO272. Article Evaluation of the free-radical scavenging and antioxidant ac...
Evaluation of Antioxidant, Free Radical Scavenging, and Antimicrobial Activity of Quercus incana Roxb. Front. Pharmacol., 23 November 2015 | https://doi.org/10.3389/fphar.2015.00277
Article Evaluation of Antioxidant, Free Radical Scavenging, and Anti...
In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Research Notes volume 8, Article number: 621 (2015).
Article In vitro antioxidant and free radical scavenging activity of...
There are several published research papers that gives protocol for the analysis of free radical scavenging activity assay (as mentioned above). As per your lab set up and research objectives, you can go through aforesaid links and conduct the experiments.
Good luck
Dear Navdeep Singh
You can detect melanin polymers with pyrolysis GC-MS. You can check the attached article. I have been looking for teams to collaborate on this issue for a long time. I wish you good luck.
Hope these articles help you:
Article Comparison of Antioxidant Activities of Melanin Fractions fr...
https://www.scielo.br/j/cta/a/4YSbqN4xNc5GVkZkjRzctFF/?lang=en&format=pdfArticle Methodology for Evaluation of Melanin Content and Production...
Article Melanin quantification: in vitro and in vivo analysis via ne...
Good luck
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