Proteins that denature at pH range 6-8?

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What is the recommended method for quantifying algal cover on a rocky shore?

I want to describe the abundance of several algae genera in the intertidal zone, on a mostly rock beach. I cannot use any destructive method (weighing or similar). Checking the percent cover in...

30 January 2024 9,981 2 View

Sensors in Formula student racing?

Hello, as part of formula student car racing I need a SLAM algorithm that will recognize cones and will build a map (there will be a few laps therefore map is needed for optimal path). The cones...

03 September 2023 1,733 1 View

What is the best soil moisture sensor for research?

What's a good device that can give an accurate and quick read of soil moisture, at the soil surface? Preferably something relatively immediate, to use in fieldwork, not an iButton or similar. Or...

15 December 2022 5,515 3 View

Minorities' attitudes toward other minorities?

I am conducting research on minority teachers and wondering what to expect with regards to minorities' attitudes toward other minority groups. Will they be more tolerant? more prejudiced? the...

20 February 2020 5,385 3 View

Are table of contents and indexes of a monograph considered as copyright matter? can we display this matter while presenting a book introduction?

some libraries show the title image and table of contents etc of a book on catalog searching. I want to ask that if any library shows title image, table of contents, indexes of a book then is it a...

08 June 2017 1,121 12 View

Has anyone good advice on what to do with a sample characteristic table that seem too big?

please see the attached table. Should I give less detail? Do the journals have graphic solutions?

19 February 2017 4,941 1 View

Why has Repression-Sensitizaion research stopped?

I want to explore if people receiving a diagmosis look for information about it or try to repress the diagmosis. I remembered studying about Repressors and Sensitizers. but when I search for...

12 April 2016 3,605 0 View

How would you recoomend measuring parents' compliance to their children diagnostic process (ASD)?

I thought of behavioral indicators such as number of cancelled appointments, times late, the length of the assessment process, number of clinicians (second opinion). I would greatly appreciate...

19 March 2016 9,770 5 View

Anyone knows of any surveys of parents and students expectations from school?

Hi I'm looking for broad research that looks at what parents and students expect from school and from teachers. Do they expect the school to make the students better people? prepare them for...

12 March 2016 3,090 2 View

Does anyone know any good works on teachers' experience of negative parental involvement?

I am conducting some qualitative research regarding teachers' perceptions of parental involvement where a lot of anxiety is surfacing from teachers regarding what they see as parental negative...

09 October 2015 2,845 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View