13 Questions 1 Answers 0 Followers
Questions related from Gehan Abdelaziz
In my qPCR experiments comparing gene expression between diseased and normal cells, I initially observed similar Ct values across two biological replicates. However, in the third replicate, the Ct...
22 May 2025 337 1 View
We are testing the effect of inhibition of proteasome on the protein level of p27 in diseased cells Surprisingly, the protein level of p27 not only is not accumulated but is even lower than in...
28 January 2025 7,503 0 View
I forget to add mercaptoethanol to my SDS sample buffer of my protein sample. I already heated my sample and stored it. What should I do ?
23 November 2024 1,005 1 View
Hello everyone, I'm studying cell cycle regulation in diseased MSC,I did BrdU cell cycle analysis,and there was significant delay in G1/S phase in diseased cells in comparison to normal cells I...
01 January 1970 5,638 1 View
Dear Researchers, I’m currently working on a project and I am interested in the subcellular location where the protein degradation occurs. Some studies, suggest that nuclear ubiquitin–proteasome...
01 January 1970 6,396 0 View
How can impaired proteasomal degradation in diseased cells be validated using functional, biochemical, and molecular assays?
01 January 1970 3,946 0 View
I’m trying to count and calculate the percentage of positive stained cells in ratio to all stained nuclei (DAPI) in a immunofluorescence staining experiment. Can I use image J software for the...
01 January 1970 7,041 3 View
I did western blot for the cytoplasmic and nuclear protein with molecular weight 20 I got good cytoplasmic band but the nuclear band was not clear as it shown in the picture although lamin A/C...
01 January 1970 2,420 1 View
How can I determine if the protein in my diseased cells is degraded by the ubiquitin dependant proteasome or by the ubiquitin independent proteasome
01 January 1970 2,429 1 View
I'd like to ask are there a reliable methods to find if protein degradation by proteosomal system is inhibited due to ER stress Which pathway or signal should I check first ?
01 January 1970 2,927 1 View
Hi everyone, I would like to check whether the ER stress response is activated in my diseased cells Could you suggest which ER stress marker I should evaluate first ? And are there any other...
01 January 1970 7,669 0 View
Could you suggest a suitable method for isolation of extracellular matrix of bone marrow mesenchymal stem cell and how can I reseed another type of cells on it?
01 January 1970 9,005 0 View
I'd like to ask is there any methods that I can confirm that the proteosomal system for p27 degradation in my diseased cells is malfunction, I already cultured the cells with epoxomicin and used...
01 January 1970 885 1 View
