Do you feel a journal on Legume Science is required?

We are planning to launch a new journal from a reputed publishing house: Journal of Legume Science. It will cover chemistry, processing and application (food and non-food). We are inviting...

08 September 2017 1,192 7 View

Can someone from India and Turkey advise me which lentil variety is mostly exported?

We are working on lentils for various processes. Mostly lentil samples are coming from India and Turkey. I appreciate if someone can provide the name of the commercial varieties of lentil samples...

10 November 2015 4,041 2 View

Can anybody comment on which particle size measurement is the best for powdered food? solid powder or in in dispersion?

During our particle size measurement using Malvern Mastersizer 3000, we measured both solid and in liquid and found liquid shows smaller particles and results are significantly different from each...

01 February 2015 1,735 10 View

How is the pore size of wheat pita bread affected by the incorporation of barley flour?

Generally gluten is affected by addition of other flour in a dough. We would like to know the effect of beta-glucan enrichment into a dough system on pore size of pita bread.

03 April 2014 6,489 2 View

Would you like to publish in Glass and State Transitions in Food and Biological Materials (Wiley)?

We (Drs. Y Roos, MS Rahman, S Sinharoy) are editing a book on Glass and State Transitions in Food and Biological Materials. We are looking for contributors having excellent knowledge on the...

02 March 2014 4,196 4 View

Thermal properties of pumpkin flour.

31 December 2012 1,638 6 View

Contribution in a book by specialists

01 January 1970 5,235 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View