Has any idea of protein profiles in desert truffle?
We are planning to launch a new journal from a reputed publishing house: Journal of Legume Science. It will cover chemistry, processing and application (food and non-food). We are inviting...
08 September 2017 1,308 7 View
We are working on lentils for various processes. Mostly lentil samples are coming from India and Turkey. I appreciate if someone can provide the name of the commercial varieties of lentil samples...
10 November 2015 4,169 2 View
During our particle size measurement using Malvern Mastersizer 3000, we measured both solid and in liquid and found liquid shows smaller particles and results are significantly different from each...
01 February 2015 1,878 10 View
Generally gluten is affected by addition of other flour in a dough. We would like to know the effect of beta-glucan enrichment into a dough system on pore size of pita bread.
03 April 2014 6,617 2 View
We (Drs. Y Roos, MS Rahman, S Sinharoy) are editing a book on Glass and State Transitions in Food and Biological Materials. We are looking for contributors having excellent knowledge on the...
02 March 2014 4,332 4 View
During thermal scanning of dried pumpkin flour, a sharp melting point was noticed at 158°C. I am not sure whether this is contributed to by lipids or protein, do you have any suggestions?
31 December 2012 1,775 6 View
Our edited book ‘Advances in Food Rheology and Its Applications’ (Woodhead Publishing/Elsevier) 1st Edition has well received by academicians and industries. We, the editors (Jasim Ahmed, Pawel...
01 January 1970 5,373 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View
