I am involved in one pot synthesis of silver and ZnO nanoparticles. I found that a protein (33 kDa) of fungal origin can perform "dual" function of synthesis and stability of nanoparticles. I have successfully characterized the protein and it's complete nucleotide, amino acid and structure is available in our laboratory.
Presently, I am focused to develop a deep understanding of “how this protein is performing dual function” and to elucidate the plausible interactions between the protein corona and a nanoparticle. In our laboratory, we are implementing both "in silico" and "experimental" approaches to understand the concept.
Being a biotechnologist, I am looking for support and guidance from experts of various other fields. I will feel immense pleasure to discuss and disclose more information in this regard., if someone is interested.
Looking for interests and contributions.
Dear L'Hocine, As Navin stated, there are various levels to explain toxicity of nanoparticles. Keep in mind that these may also include apoptosis, necrosis, or a combination of both. I would also add that there may be effects on cell morphology, which we have characterized for example using the parameters of nuclear area factor and cell area factor. let me know if you are interested and I can link you to some of our publications.
I am working on gold and silver nanoparticles and their interactions with IgG and IgY.
We are developing anti-biolfim coatings based on nanoparticles and highly interested to your work. How to predict and/or the toxicity mechanisms of nanoparticles ?
Dear L'Hocine Yahia,
Sorry for late response....
The prediction of toxicity of nanoparticles can be performed at various levels....genotoxicity, cytotoxicity, sub-cellular, etc..
Plz check the following most relevant article in this regard
The toxicological impact of nanoparticles by Roberta Brayner. Nano Today FEB-APR 2008 3:48-55.
Most predictive mechanisms involve ROS estimation and determination of ionic dissociation of nanoparticles. If you wish, I can look forward to help you in this regard at experimental levels.
Waiting for a positive response.
Dear L'Hocine Yahia,
Sorry for late response....
The prediction of toxicity of nanoparticles can be performed at various levels....genotoxicity, cytotoxicity, sub-cellular, etc..
Plz check the following most relevant article in this regard
The toxicological impact of nanoparticles by Roberta Brayner. Nano Today FEB-APR 2008 3:48-55.
Most predictive mechanisms involve ROS estimation and determination of ionic dissociation of nanoparticles. If you wish, I can look forward to help you in this regard at experimental levels.
Waiting for a positive response.
Dear L'Hocine, As Navin stated, there are various levels to explain toxicity of nanoparticles. Keep in mind that these may also include apoptosis, necrosis, or a combination of both. I would also add that there may be effects on cell morphology, which we have characterized for example using the parameters of nuclear area factor and cell area factor. let me know if you are interested and I can link you to some of our publications.
How to prepare nanoparticles of various salts using chemical precipitation
Dear Navin,
Thanks a lot for your input and also for the very nice paper. I will get back to you after the Holidays. Best wishes.
I do not seem to understand what your question is. Are you asking how to evaluate the ability of your protein to support the synthesis and to bring stability to your nanoparticles? Do you mean you put the protein in the pot together with the materials needed to make ZnO and silver nanoparticles? Do you mean to say that when the protein is not present, the nanoparticles do not form? Or do they form but they do aggregate? Please explain so that I can have more details to work on to help you.
There are many different interrractions, e.g. AuNPs can transfer electrons form active site of the enzymes:
Article Spectrophotometric evaluation of gold nanoparticles as red-o...
Dear Zordia,
The explanations of your doubts are as follows:
My experiment strategy involves a reaction mixture comprising of a protein and precursor ions (may be Ag+ or Zn+2). Not in combination.
Yes, i mean to say that protein is solely responsible for synthesis as well as stabilization. the protein itself is working as a reducing agent as well as stabilizing molecule. Of course, there is no nanoparticle synthesis in absence of protein as no other reducing agent is used in my experiments.
I am deeply interested in studying the mechanism involved in nanoparticle formation and stabilization with major emphasis on protein nanoparticle interactions.
I hope I have cleared your doubts. Looking forward to hear from you.
Regards
Navin Jain
Protein-Nanoparticle interaction could be exploited for creation of 'functionalized' nanoparticles for various purposes, e.g. analytical purposes, an example in attached publication.
Article Magnetic Gold Nanoparticles in SERS-Based Sandwich Immunoass...
Proetin (enzyme) induced nano[article formation:
Article AFM Study of Conducting Polymer Polypyrrole Nanoparticles Fo...
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