I am interested in the separation of two proteins of 33 and 31 kDa. Can size exclusion chromatography be a useful tool?

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Steingrimur Stefansson

If both proteins are globular, I don't think there is any chance of separating them using standard SEC.

Navin Jain

thanks for response.......as both proteins are globular in nature, it's difficult to separate from SEC......Can u plz suggest me any other chromatography method????

Try running the proteins on native PAGE. If they separate well there you can try ion exchange.

Anirban Roy

Its really tough to separate so closely related proteins. Dr. Stefansson is right...ion exchange can help in this regard.