RNA isolation from fungus.

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I am interested in the separation of two proteins of 33 and 31 kDa. Can size exclusion chromatography be a useful tool?

Protein purification strategy.

01 February 2013 2,065 4 View

How to inhibit/prevent fungal "self" RNAses activity during RNA isolation?

During RNA isolation from fungus, most of the reactions result in smear formation.

01 February 2013 1,445 2 View

Protein-nanoparticle Interactions

I am involved in one pot synthesis of silver and ZnO nanoparticles. I found that a protein (33 kDa) of fungal origin can perform "dual" function of synthesis and stability of nanoparticles. I have...

11 December 2012 6,802 14 View

How to prepare silver ions (Ag+) for metal-protein docking studies?

In order to determine the binding site of silver ions (Ag+) in a protein structure, I am planning to perform docking studies (metal-protein docking). However, I am not able to prepare the ligand...

11 December 2012 6,588 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Deepak Pakalapati

Please provide the details of the kit and protocol used. That would be useful 4 troubleshooting ur question (either low yields or degradation)

Navin Jain

Thank you sir...I am using Qiagen Plant Mini kit....I feel the problem is the presence of high amount of fungal "self" RNAses.

Pls C Pg-14 of this attached document. Is that what u did??

What buffer did u use RLC or RLT? I remember, pradeep used RLT for Cyanobacteria after sonication lysis . A view of the gel pic would help.

Himanshi Kapoor

I think you should not go for kit based extraction for RNA, try extracting it manually using Triazol method.