i need to know mutations can effect on protein backbone ,protein folding,secondary structure ?
how to study a certain type of mutations with another type of protein (not mutation and totally different from mutation) by (bioinformatics tools). What is your opinion and suggestion about this?
05 June 2019 4,401 2 View
i want study cancer genome by bioinformatics tools. can advice me in articles or review paper can guide me to do my research.
03 April 2019 5,164 5 View
What kind of scientific research ( hypothesis ) can I do and complete it in the absence of availability or absence possibilities to do this research. Knowing the research I want to do is related...
08 September 2018 6,374 5 View
I want to study of 3D protein structure (contact map) with complex networks, calculate the centralities of C alpha atoms, specially those atoms of the amino acids, in which software or tools can ?
09 October 2015 9,055 2 View
i run MD simulations with GROMACS for my protein and i want to know is it my simulations converged. How to know that ,how i will do it and confirm ?
09 October 2015 8,375 1 View
In which file i can find c alpha atoms for my protein in between GROMACS files?
09 October 2015 8,718 0 View
Im trying to run x.pdb file but when select force field it give Error(Residue not found).already tried different force field but keep same error. what can to do to solve this error?
08 September 2015 6,685 7 View
Im using molecular dynamics to study stabilization of protein at low pH but when am checking by VMD it shows no any salt bridges also I was used different software like gromacs salt bridges but...
05 June 2015 8,199 5 View
I want to know n terminus and c terminus for protein but according to molecular dynamics simulations by gromacs especially in (RMSF).
11 December 2014 6,275 3 View
I did molecular dynamics simulations for different mutations and I want to compare how hydrogen bonds effects on stability and the other properties of mutations.
05 June 2014 5,447 3 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View