How to study mutations with another type of protein by bioinformatics tools?

how to study a certain type of mutations with another type of protein (not mutation and totally different from mutation) by (bioinformatics tools). What is your opinion and suggestion about this?

05 June 2019 4,186 2 View

How to cancer genome analysis by bioinformatics tools?

i want study cancer genome by bioinformatics tools. can advice me in articles or review paper can guide me to do my research.

03 April 2019 4,952 5 View

Absence of availability or absence possibilities to do biological and medical science ?

What kind of scientific research ( hypothesis ) can I do and complete it in the absence of availability or absence possibilities to do this research. Knowing the research I want to do is related...

08 September 2018 6,010 5 View

Which tools and software to study of 3D protein structure (contact map) with complex networks?

09 October 2015 8,843 2 View

How simulations converged?

09 October 2015 8,187 1 View

C alpha atoms GROMACS?

In which file i can find c alpha atoms for my protein in between GROMACS files?

09 October 2015 8,455 0 View

What would the reason be for residue not found when start to run simulations with Gromacs?

08 September 2015 6,476 7 View

Anyone familiar with modeling salt bridges at low pH?

05 June 2015 7,998 5 View

How to determine n terminus and c terminus in molecular dynamics by gromacs?

11 December 2014 6,041 3 View

Can anyone help me with how to analyze the effecting of hydrogen bonds?

I did molecular dynamics simulations for different mutations and I want to compare how hydrogen bonds effects on stability and the other properties of mutations.

05 June 2014 5,223 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View