The current is way too low. It should be more like 50-100 mA. There may be a problem with the electrical connection, there may not be enough buffer to cover the top electrode and the gel, or the running buffer may have been prepared incorrectly.

You need to prepare everything again. Something is obviously wrong. 

so much thanks Mr.Gonzalez.

i'll try it again with new prepared materials. I hope it works

The current is way too low. It should be more like 50-100 mA. There may be a problem with the electrical connection, there may not be enough buffer to cover the top electrode and the gel, or the running buffer may have been prepared incorrectly.

You must check the preparation of buffer. Is the marker migrated ?

 thanks a lot Mr.Shapiro. but the buffers were enough and they covered gel.and about buffer,i made it 2 month ago and it's been keeping in 4 c.also i did SDS-PAGE before with this buffer and it worked. by notice to the markers which they didnt run too, i think  you are right and  may be electrical connection has problem.

thanks Mr.Gouda. but markers didnt migrate,too.

You are most welcom.

You can check the buffer, voltage, connections etc.

I had the problem like this but a bit different. In my case, though protein was well migrated in staging gel, there was not sufficient separation of protein in the SDS-page gel below staging gel. After checking all systems of Western blot arrangement, we found that pH meter was not measuring correct pH of buffer solution we had prepared for SDS-page electrophoresis. Thus pH of the buffer was not exactly what we had presumed for electrophoresis. That's why we suffered problem during electrophoresis in SDS page gel and protein was not well separated along with protein marker, particularly below 45 kDa. So I would suggest being confirmed about the pH of the buffer used in electrophorosis. Another factor, temperature also plays a vital role in protein separation during electrophoresis. Try to maintain a lower temperature than room temperature. I used to put the western blot arrangement in an ice chamber so that high heat would not be produced in the SDS-page gel and distinct separation of protein would have appeared. For detail of the problem type I had faced regarding protein migration in SDS-page electrophoresis, please go through my researchgate question section. Good luck. 

Did you denatured your proteins at 95 degrees?

I agree with prof. Shapiro, the problem is either in the buffer or in the electical connections, since usually the current should be around the values he suggested.

apparently the buffer is not working. make new running buffer and then check the value of current while running the gel. if it is between 50-100 mA at that voltage u applied, the protein should migrate. it is a good habit to make fresh buffer after 2-3 uses. the conductivity of buffer decreases after repeated usage.

thanks a lot.Mr.Siddiqui. i'll try them definitely, as you say.

thanks to all.and special thanks to prof.Shapiro.

according to your helpful tips, i found the problem is the electrical current. after solving the current problem, i ran my samples once more without any problem.