Protein degradation

More Sandeep Talapatra's questions See All

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

Method to measure printed layer thickness on paper substrate ?

I want to measure thickness of screen printed graphene ink on paper substrate. Can anyone suggest any accurate and feasible method to measure it. I tried profilometry (stylus & optical) but...

11 January 2021 6,179 6 View

For leadership in creativity and innovation , happiness enhances creativity, but the opposite of it - sadness - ?

for leadership in creativity and innovation , happiness enhances creativity, but the opposite of it - sadness - ? if one is neutral to happiness and sadness, will he be able to lead...

27 December 2020 4,744 5 View

Does Cheer encourages Creativity or Creativity encourages cheer ?

Does Cheer encourages Creativity or Creativity encourages cheer ? Cheer and creativity. please do write your answer.Happy new year to all of your .

27 December 2020 1,302 5 View

Is it good to increase false citation?

Nowadays, I have seen many researchers are involved in increasing their citation count. They are using various inappropriate approaches for that. However, in last they have a good number of...

16 December 2020 2,484 5 View

What are the best markers for Erythropoiesis in mice?

What are the best markers for Erythropoiesis in mice? There are several markers like CD45, CD34, CD117, CD105, CD235a, CD71 based on specific stages of erythropoietic cells. Which of the above or...

19 October 2020 3,303 8 View

Mice Duodenal Enterocyte marker ?

Hi, what is the best Duodenal Enterocyte marker for 1. Immunohistochemistry 2. Western blotting 3. Flow-cytometry/FACS

19 October 2020 5,400 3 View

Is it possible to find out the in-plane lattice constant for a polycrystalline film without doing in-plane XRD and Reciprocal space mapping?

I am trying to calculate strain in GeSn system. From theta-2 theta XRD scan, the films turn out to be polycrystalline in nature showing three slightly shifted peaks (with different concentration...

14 September 2020 3,602 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View

Sandeep Talapatra

Thanks but I always use protease inhibitor cocktails and also pmsf but that doesnt help either.

can you please suggest me something else like any protocol or method which I can try

Seyed Mohammad Gheibihayat

To avoid proteases in good condition you can do the steps of purification in cool temprature