Propidium Iodide Reconstitution?

04 March 2020 1 9K Report

So I received 10mg Propidium Iodide and want to reconstitute it in ddH2O, but wanted to inquire about what concentration I should use. I've read that I should dilute 1mg/1ml for a 1.5mM solution. Would it be an issue if I made it more concentrated, and diluted 10mg/1ml for a 15mM solution?

Vipul Batra

No issues. Rather, it is good strategy to make stock and then aliquots and then prepare working solutions out of it.

Badges
Science method

More Jacob Klemm's questions See All

Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

How to simulate a metalens with backward propagating source using a Lumerical FDTD?

Dear Sir/Madam, I am currently working on a beam shaping problem and have referenced the Metalens example provided on the Ansys Lumerical website. In that example, a forward propagating plane wave...

14 June 2024 4,333 0 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

Protocol for Coating Plates with Collagen?

Does anyone have guidance on coating 96-well plates with collagen-I? Looking for a protocol. We are using Corning® Collagen I, Rat Tail, 100 mg.

22 April 2024 6,835 1 View

How do I determine and what radical is this in this EPR graph (DMPO)?

So I'm trying to determine what radical this is captured with DMPO. How do I determine and calculate what radical this is? Thanks!!

06 April 2024 7,084 3 View

Is it required to send interview transcriptions for publishing qualitative research in a journal as part of data availability?

As someone engaging in ethnographic research, are we expected to disclose transcribed data to the journal where we would like publish an article?

03 March 2024 3,653 3 View

Which Promotor Sites can I use for In Vitro Transcription?

Our lab is trying to use in vitro transcription to create mRNA of our inserted on a pcDNA 3.4 TOPO plasmid. I noticed it does not have a T7 promotor sequence. Are there other available promotors...

26 February 2024 1,852 1 View

How should one teach ETHICS to the new generation of college students in 2024?

More and more, teaching ETHICS has become an important part of the college curriculum, but teaching it has not always been easy or up to part with the generational changes in the student...

02 January 2024 7,862 6 View

Buffering pH of DNA Binding Solutions for Silica Columns?

I am trying to prepare some custom DNA binding solutions for purifying DNA with silica columns. I have prepared one with 4 M guanidine thiocyanate, 30% isopropanol, and 10 mM Phosphate buffer, pH...

03 December 2023 2,197 0 View

How do we design a three level dimensions of organizational ambidexterity?

I am currently exploring on the concept of "Organizational Ambidexterity". I would love you give me an insight on the way to design its dimensions in a three level scale, where it will depict its...

27 October 2023 3,058 3 View

How to represent Annexin V apoptosis study data in publications?

Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...

13 July 2024 356 3 View

The proocol of Anexin V Alexa Fluor 488 and Propidie Iodide for bull semen?

Hello Everyone, I will use Annexin V Fluor 488 and propidium iodide for the analyze of frozen thawed bull semen.If anyone is familiar with using these fluorophores, can they share their flow...

14 May 2024 4,241 0 View

Has anyone seen vesicles when treating primary RPE cells?

We have been working with RPE cells in our lab. In both the cell line and the primary cells we work with, we consistantly see vesicles appear post treatment. We have stained them with propidium...

15 February 2024 3,608 0 View

How much time does it take to kill fungal cells with amphotericin B?

Hi, all. We are trying to evaluate the resistance to amphotericin B using budding yeast as a model system. I am wondering if anyone know that how long does it take for ampB to kill a wt yeast...

03 January 2024 4,095 0 View

Which solvent different from PBS 1X do you recommend for keeping cells alive for 30-60 min?

Im evaluating cell viability in flow cytometry with propidium yordurate and I need to keep my cells alive in a solvent that can keep them up to 1 hr and can also be used to be injected in the flow...

18 December 2023 3,969 2 View

Why do A431 cells form clumps in propidium iodide staining solution?

I've been trying to study cell cycle using PI (propidium iodide + Triton-X + sodium citrate)b staining solution. I trypsinize my cells (A431: skin carcinoma), fix them using 100% methanol and...

20 November 2023 2,443 3 View

What should be the final conc of PI in apoptosis/necrosis assay with annexin V in flow cytometry?

What should be the final conc of PI used?

11 October 2023 587 2 View

Why does IP+ population "shift" in AV-IP dot plots?

I have a recurrent issue when analizing apoptosis by flow cytometry using Annexin-Propidium Iodide staining. I can fairly compensate each dye, but when I put the double stained positive control...

03 September 2023 820 8 View

How do I stain my MCF-7 spheroids for live-dead assay?

I have been trying to stain my spheroids for live-dead assay with Calcein-AM and Propidium Iodide and the spheroids are either not taking up the stain or they are breaking apart. I need help...

27 August 2023 286 0 View

If bacterial cells are completely lysed following treatment with an antimicrobial- then washed- then PI stained- Why is concentration lower?

Hi- I have analysed some PI stained samples through flow cytometry. The results show a much lower concentration of cells in those samples which have been treated than those which haven't...

24 July 2023 7,453 2 View