Im evaluating cell viability in flow cytometry with propidium yordurate and I need to keep my cells alive in a solvent that can keep them up to 1 hr and can also be used to be injected in the flow cytometry.
Hello Lucas,
Use HBSS (Hanks' Balanced Salt Solution) with calcium and magnesium, which is a balanced salt solution with trace amounts of glucose.
Cell viability is not compromised while cells are in HBSS since it is a balanced salt solution with glucose and it maintains osmolality and physiological pH. Cells will remain relatively happy and survive for at least 2 hours in HBSS.
So, you may use HBSS instead of PBS, and HBSS can also be used in flow cytometry.
Best.
Hello,
I have used Ringer's solution supplemented with glucose for this purpose. This keeps cells happy for a while and does not cause activation of autophagy or cell death, but keep in mind that you still need to keep cells warm during the staining process.
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