Promega FuGENE HD transfection reagent to transfect HCC827 cells?

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Transwell migration assay for TNBC?

I wish to perform transwell migration assay for checking the effect of a drug on the migratory properties of Triple Negative Breast Cancer cell line (MDA MB 231). Would a difference in FBS...

06 April 2024 8,691 2 View

What should be the PCR profile of full length gene amplification with amplicon size more than 1.5 kb?

I am facing problem to amplify full length gene (around 1.5 kb) using cDNA with primer length varying from 24-28 decamer( attached with adapter) at tm 62 degree celsius. What PCR profile I should...

07 December 2023 8,199 3 View

What is the procedure to add 2 restriction sites to primers for cloning a complete gene sequence and is it necessary to add setting sequence ?

how to add two restriction enzyme sites to primers for cloning a gene sequence (size 1-2kb) to pGEM-T Easy Vector followed by pcambia 1301 and is it necessary to add setting sequence before the...

20 September 2023 7,443 4 View

Writing tools for a thesis?

what are the most useful and effective writing tools for the writing of a thesis?

10 August 2023 2,657 4 View

Is there any method or tool or instrumental approach to prove or explain experimental data of enzyme activity enhancement by metal ion?

I am working on some commercial protease enzymes to enhance their activity by different chemical agents. I found some metal ions have the ability to enhance the proteolytic activity up to 20 to...

25 April 2023 9,435 2 View

What is the Physiological salt concentration of the cell also inside nucleus?

I am actually performing an Md Simulation study of the protein+DNA complex and using Amber99 ff. but I am confused about what concentration of salt to take for the simulation.

24 October 2022 4,549 1 View

Fowler Guggenheim isotherm calculation when theta > 0.6?

Ayawei 2017 mentioned using Fowler Guggenheim isotherm is only valid when theta is less than 0.6. What can be done to overcome this limitation?

14 July 2022 8,861 0 View

How can I model dynamic contact angle of rolling droplet in OpenFOAM?

about droplet impact and droplet rolling on a superhydrophobic plate by OpenFOAM, I couldn't model dynamic contact angle as a boundary condition. could anyone help me with that problem?

24 June 2022 6,006 0 View

How can we export structure's image from CST Studio Suite?

I want to add the picture of my simulated structure into word but I don't know how to export the image from cst? is there any way? Thank you so much

05 January 2021 7,302 4 View

For electrochemical capacitors which type of specific capacitance is more technically preferable to represent?

Capacitance of electrochemical capacitors generally represented in relative scale, i.e. the units are represented either related to electrode area or the mass of active material. In most of the...

22 June 2019 8,383 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View