I wish to perform transwell migration assay for checking the effect of a drug on the migratory properties of Triple Negative Breast Cancer cell line (MDA MB 231). Would a difference in FBS concentration in the upper and lower chambers of the inserts act as sufficient cue to promote cell migration?
Hello Titas Raha
Yes, serum is often utilised in transwell migration protocols to provide chemotactic stimuli. The cells will tend to move towards nutrient-rich media which is at a higher concentration of FBS. For this purpose, a chemoattractant gradient may be created by placing cells in the transwell insert with lower serum concentration supplemented media. Cells will tend to migrate towards the source of higher FBS concentration added to the lower chamber.
You may want to refer to the article attached below.
https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11122#:~:text=Serum%20is%20often%20utilised%20in,et%20al.%2C%202016).
Best.
Yes, a difference in Fetal Bovine Serum (FBS) concentration between the upper and lower chambers of the Transwell inserts can act as a sufficient chemotactic cue to promote cell migration. The principle behind the Transwell migration assay is that cells will move from an area of lower concentration of a chemoattractant to an area of higher concentration.
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