Proliferation inhibitor for investigating cell migration?

More Nejra Cosic Mujkanovic's questions See All

What is the most important technology that can improve the security of the medical Internet of Things in the future?

Although the application of IoMT technologies to healthcare brings many benefits, it also brings great security challenges. Other IoT devices are also exposed to security threats, but the...

17 February 2023 770 7 View

Confocal microscopy image quantification?

Hi, I transfected HEK cells with fluorescent ABCG2 transporter (multidrug resistance transporter) , it has a GFP probe attached, and I am performing a live cell confocal microscopy experiment,...

21 September 2021 4,691 2 View

Whether and to what extent the COVID-19 pandemic affected the rise of information literacy?

In the age of the COVID-19 pandemic, people are forced to use information technology more than usual.

27 May 2021 6,927 4 View

Problem with cell growth?

Dear all, I have a very strange problem with my cells (HMLE cells with empty plasmid pBP). They are growing well in the flask, but when I seed them on a 6 or 96-well plate, they don't look very...

23 November 2020 10,045 5 View

ROS detection with DCFH-DA and MitoSOX?

Hi, I am measuring the effect of some compounds on cellular ROS level in HCT116 cell line, and I am wondering is it possible that I detect the ROS increase by MitoSOX, when treating the cells...

15 May 2018 5,061 4 View

Stability of glufosate ammonium on high temperatures?

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17 March 2016 9,695 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

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09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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Jack Hopkins

Hi Nejra Cosic Mujkanovic

A low concentration of mitomycin C may suit your experiment - here is a paper detailing the experiment in A549 cells: Article In vitro effects of mitomycin C on the proliferation of the ...

Alternatively, you could use thymidine to block DNA synthesis at the G1/S phase boundary

Article Cell Synchronization by Double Thymidine Block

Good luck with your experiments.

Nejra Cosic Mujkanovic

Thanks Jack! I will try this :)