We are working with micropropagation in a capparidaceae family and I had problems with bacterial and fungal contamination when using shoot apex. I already tested twine 20 and alcohol 80%, sodium hypochlorite in several concentrations and times and mercury chloride 0.05% and 0.1%. Also left the plants washing with running water before the disinfestation for 20 minutes and still contaminated.
Have you tried any of sterilization methods mentioned above stirring at 300-500 rpm with a magnetic stirrer?
Had u ever tried to used the plant that grown in vitro? or, try to use 95% alcohol... Or try to add antibiotic in the media (find the concentration, i never use it myself)
I think H2O2 (1-2 ml/l) add it as filter sterilized after autoclaveing the medium. This treatment works with smoe woody species
- Badges
- Science method
- Similar topics
- Biological Science
- Ecology