Why no gene deletion in S. cerevisiae using Lox p system?

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Dear all, Is there any model available to predict the "possible diseases" based on the available pollutant concentration in air ?

In detailed human health risk assessment models. Is there any model that can predict the possible disease.

21 December 2020 7,535 5 View

How to get best LSTM model for regression problem? how to tune or select hyper parameters?

How to get best LSTM model for regression or prediction problem? how to tune or select hyper parameters?

29 June 2020 9,377 4 View

How to work with phasor model in matlab?

I am running IEEE 13 bus model in MATLAB with discrete time . But when I connect Renewable energy DFIG model with IEEE 13 bus system not working due to phase model of DFIG. How to rectify error?

31 January 2020 7,950 4 View

In fluent heat flux analysis results are not converging ?

I had solved the heat flux analysis of the multi-domain problem in fluent. Even after the 20000 iterations also the solution is not converging. As the iterations increase the results are diverging...

05 November 2019 1,713 3 View

Any specific procedure to be followed while converting one dimensional data into image?

Is there any specific procedure to convert 1D data to image before feeding convolutional neural networks ?

18 October 2019 8,309 4 View

Is there any open source Software In Loop (SIL) test method.?

Is there any open-source Software In Loop (SIL) test method available to test the network that was actually created in MATLAB. Anyone guide or give reference to use OPAL-RT testbed to integrate...

06 October 2019 8,124 1 View

Do addition of nanopartilces in soil increases the Plant yield ?

Can any one explain me that addition of nanoparticles enhances the growth of plant and produces more yield. If so, can anyone list me the nanoparticles i can use Plant is Tomato and Aubergines

24 June 2019 5,402 3 View

Do anyone have working protocol for cardiac perfusion in mice?

I am new to perfusion. I need to do cardiac perfusion in mice and my area of interest is brain further to sectioning and IHC. Does anyone have standard protocol of cardiac perfusion for this. Help...

29 May 2019 6,919 3 View

Is addition and removal of Co-author during submission of revised article is acceptable @Elsevier ?

I like to know the removal, addition and shuffling of co-authors is against moral and ethics in Elsevier Journals. Is it necessary to contact a editor to make such changes

20 February 2019 3,676 18 View

How to gelatin coat flasks for primary rat astrocyte culture?? Suggest me with how much percentage gelatin should i use??

I'm gonna culture rat mixed glial cells for first time. suggest me with how much percentage gelatin should i use to culture cells..

07 November 2018 7,023 6 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

How many days do we need to detect adoptively transferred Treg in the tumor site in tumor-bearing recipient mice?

Hello, I've got a plan to isolate mouse splenic Treg (WT and KO of target gene) and inject these CD45.2 Treg into tumor-bearing CD45.1 mice. My question is how many days do I need to wait to see...

11 February 2021 8,238 1 View

Chanuka Kulatunga

What is the promoter for Cre in your vector ?

Thiyagarajan Sekar

GAL1 promoter and I have used different Galactose concentration from 0.5 to 4 %.

Alejandro De Las Peñas

I am not famliliar with the LoxP system, but PCR a marker with Fw and Rv primers complimentary to the gene to be knockout, works out pretty well.