How to model the vapor plume during laser welding process using Fluent?

Problem description: I am using ANSYS Fluent (multiphase VOF & solidification and melting model) to simulate laser welding process. Is there any possibility of establishing a fully coupled...

05 November 2020 893 1 View

Should I add penicillin/streptomycin into cell culture media for doxycycline inducible system?

Hello, since I have no experience in Tet-on system in drosophila S2 cell culture, I need some technical help... Should I add P/S into the cell culture media? What's the doxycycline concentration...

17 September 2020 7,497 5 View

Why there is a 11 kDa band showing in SDS-PAGE of my IgG1 digestion with Papain?

Hi guys, I am using the papain digestion system to abtain a Fab. Here is my recent result, I got thin bands around 55, 45; thick bands around 30, 25, which may indicate incomplete digestion, but...

27 June 2020 3,882 9 View

Black paint for supercapacitor electrodes?

Do anyone knows what kind of materials could be used to paint a supercapacitor electrode as shown in the picture (the coated black shining materials)? Any help is appreciated. Thanks!

05 March 2020 2,194 4 View

Which short versions of Ryff's Psychological Well-Being Scale do you recommend?

I want to assess the well-being of farmers in Southeast through both an assets and psychological perspective and am having trouble finding a good measurement scale for the latter. Ideally, I want...

03 January 2020 1,927 3 View

What type of brain cell or tissue is this?

We recently discovered a probe could stain brain tissues and showed a very specific distribution, wondering what type of cells or tissue is this?

03 March 2019 7,214 6 View

Hey, does anybody know if there are some bacteria can use acetate to produce methanol or methylamine?

any bacteria can use acetate to produce methanol or methylamine? or use H2 and CO2 to produce methanol?

15 May 2018 9,401 5 View

Any one who is working on or interested in vision problems on videos?

I'm very happy to learn, discuss and collaborate on some vision topics on videos. If you are also very interested, feel free to contact me.

10 May 2018 4,869 2 View

In Abaqus what is the most efficient way to add wire feature

08 July 2017 8,539 5 View

T lymphocyte apoptosis in mice

We have found that deleting a transcription factor in Tregs causes fatal autoimmune disorder in mice, concomitant with accumulation of effector memory CD4 cells (CD44hi CD62L low) in blood, LN and...

01 July 2017 4,423 1 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

What is the ideal value of UACI for image encryption scheme?

UACI - Unified Averaged Changed Intensity between cipher images. Why some papers refer ideal value to be 33.33 and some refer it to be 33.46? Which is the correct value? (In reference to image...

11 February 2021 9,562 2 View

Antibody fragments after Dha (Dehydroascorbic acid) re-oxidation?

Hi, I am trying to re-oxidize the disulfide bridges of an antibody with DHA. I always have some good amount of HL fragments (heavy + light chain fragment), despite varying reaction times, pH,...

31 January 2021 9,495 2 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View

What are different ways to compare the histogram of cipher images from various encryption schemes?

Is there a metric to find if one histogram of cipher image is better than other? I tried calculating the variance of the image but couldn't get the intended results. Are there ways to compare...

28 January 2021 479 3 View

How can I remove salts from crude seaweed extract (except dialysis)?

Hi, I am interested to obtain phenolic fractions from crude seaweed extracts (using ethanol) and test for their antioxidant properties. Since most of the in-vitro antioxidant assays are...

26 January 2021 3,889 5 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Who knows some kind of legal scheme for renewable energy certificates (REC, iREC, GO) integration requirements?

Who is a "central body" for renewable energy source certification and process implementation? Are REC, iREC, GO securities or there is another legal scheme? Is it possible to trade them outside...

21 January 2021 4,179 3 View