How to use prime editing (PE) to insert large fragments of genes in seaweed? How to design the specific experimental scheme and pegRNA?
Background: A Chinese company (DT) is seeking market development opportunities in Southeast Asia, the Middle East, and Eastern Europe. Before conducting business, it requires communication...
24 April 2024 6,815 0 View
I am working on pancreatic cancer. Now, I am trying to generate a pancreatic exocrine organoids using my KPC mice. But after I checking the protocol, PancreaCult™ Pancreatic Organoid Growth Medium...
20 April 2024 3,879 1 View
In photochemical system, why do the electrons produced by the photoanode spontaneously migrate to the photocathode,even when no external bias is applied?
29 February 2024 8,253 0 View
I want to specifically block a GPCR on cell membrane (also has mitochondrial distribution), but all commercially avaible drugs are cell permeable. therefore, I was seeking to use antibody to block...
17 February 2024 4,110 1 View
Hello everyone! It is my first time to fabricate InP-based photonic crystal. I've already finished fabricating the photonic crystal using EBL. And now I want to remove the 200nm SiO2 mask from...
07 January 2024 2,394 3 View
Hello experts and scholars, I would like to ask if I place a radar measuring instrument on a building to measure the vibration characteristics of a nearby building, how can I study the vibration...
12 December 2023 3,087 2 View
I really want to know what is the best timing to inject tamoxifen to induce PDAC. My preliminary experiments is not enough to give a suggestion. I have read some papers and they have different...
04 December 2023 9,269 0 View
My colleague has come up an idea of using two inserts to ligate into one vector at the same time, which is like : vector-SpeI-Insert A-BamHI-Insert B-NotI-vector (Spel-Insert A-BamHI, BamHI-Insert...
28 November 2023 846 2 View
I selected a single restriction enzyme cutting site BamHI in a plasmid of around 6 kbp, performed enzyme digestion, and carried out dephosphorylation treatment. I attempted to insert a fragment of...
25 November 2023 1,163 1 View
The APS minerals in my samples (the major composition is dickite) could be divided into two kinds, including aggregations (acicular and scale-like) and zoned structured crystals (mainly cubic)....
07 November 2023 9,759 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View
How can I select cells in prime editing without fluorescence-activated cell separation?
31 July 2024 8,551 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View
I'm currently working on gel purification in order to get DNA fragment for T4 ligation. But I have been able to take only 8.5ng/μl as a highest concentration of DNA fragment. And could not get...
01 July 2024 4,912 9 View
Please, can the fragmentation obtained from the mass spectrometry be predicted more easily using any free software? Kindly recommend it, thanks.
01 July 2024 5,327 6 View
Fermented beverages are very important drinks for some community as they consume daily with huge quantity, are traditionally believed as pharmacological and medicinal property along with...
16 June 2024 7,235 0 View
Hello, Has anyone done Long Range PCR on dried blood spot gDNAs? We are not able to amplify 12kb fragments from the gDNA. GDNA was manually extracted using ThermoFishder MagMAX™ DNA...
06 June 2024 5,667 0 View
I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...
02 June 2024 8,038 1 View
I did GCMS/MS, LCMS of my sample of interest. I got Mass fragments in ionization process. Now facing issues to get the parent molecules name/formula. Due to complexity of sample (its a mixture of...
01 June 2024 1,056 4 View