I need to store sediment samples for extraction of RNA to do metatranscriptomic analysis but the RNA extraction cannot be performed before around 6 months since sample collection and shipment. No problem with keeping at -20 or -80 °C, but is that enough or should I add RNA later or similar? I read that these chemicals don't work with sediment, is that true? any suggestions? Thanks!
Abu Sayeed Mohammad Mahmud
Go through the following link, this will definitely help you to understand about RNA purification from soil (marine sediment).
http://www.mobio.com/blog/2010/03/23/10-tips-for-the-isolation-of-high-quality-rna-from-soil/comment-page-1/
1 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA
More Susana Claudia Vazquez's questions See All
Similar questions and discussions