Susana Claudia Vazquez

6 Questions 8 Answers 0 Followers

Questions related from Susana Claudia Vazquez

Scaling and/or transformation of environmental variables before RDA analysis?

Hi I want to perform and RDA analysis using bacterial community data and environmental data as explanatory variables. I have some variables that are percentages, others are direct measures of...

22 May 2020 9,636 0 View

Normalization of sequence length for diversity indexes calculation?

Hi I need to combine two 454 datasets but one was obtained with FLX (400 nt) and the other with FLX+ (700 nt), both starting with the same forward primer. I know I have to rarefy to the lowest...

19 February 2016 4,329 3 View

Preservation of marine sediment samples for RNA extraction: how and for how long??

I need to store sediment samples for extraction of RNA to do metatranscriptomic analysis but the RNA extraction cannot be performed before around 6 months since sample collection and shipment. No...

24 July 2014 9,137 1 View

Total DNA or environmental DNA (eDNA)?

I'd like to have your opinion on this subject. When publishing a paper or writing a report or thesis, if I refer to the DNA extracted from soil samples taken from a mesocosm experiment (i.e....

01 January 1970 8,233 3 View

Does anyone have experience with library prep using the mate pair kit for Illumina sequencing usin MiSeq?

I am doing a research stay at an institute and they offered to resequence the genome of one of my strains. We have the pair end sequences but the assembly went not so good so they suggested...

01 January 1970 3,777 8 View

Best 16S V region for 16S amplicon sequencing, for both bacteria and archaea?

Which is better, V1-V3 or V3-V4 regions for 16S amplicons using 2x300 bp illumina sequencing? Are both good to target bacteria and archaea?

01 January 1970 1,559 4 View