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Questions related from Susana Claudia Vazquez
Hi I want to perform and RDA analysis using bacterial community data and environmental data as explanatory variables. I have some variables that are percentages, others are direct measures of...
22 May 2020 9,708 0 View
Can anyone explain the differeces between CCA, CAP and RDA analysis and plots and how or in which basis to choose which to use?
13 September 2019 1,519 3 View
Hi I work with soils and sediments from Antarctica, and as DNA is very stable under those low temperatures, I would like to use RNA extractions instead of DNA extractions from the environmental...
31 January 2017 6,405 7 View
Hi I need to combine two 454 datasets but one was obtained with FLX (400 nt) and the other with FLX+ (700 nt), both starting with the same forward primer. I know I have to rarefy to the lowest...
19 February 2016 4,381 3 View
I need to store sediment samples for extraction of RNA to do metatranscriptomic analysis but the RNA extraction cannot be performed before around 6 months since sample collection and shipment. No...
24 July 2014 9,184 1 View
I'd like to have your opinion on this subject. When publishing a paper or writing a report or thesis, if I refer to the DNA extracted from soil samples taken from a mesocosm experiment (i.e....
01 January 1970 8,282 3 View
I am doing a research stay at an institute and they offered to resequence the genome of one of my strains. We have the pair end sequences but the assembly went not so good so they suggested...
01 January 1970 3,820 8 View
Which is better, V1-V3 or V3-V4 regions for 16S amplicons using 2x300 bp illumina sequencing? Are both good to target bacteria and archaea?
01 January 1970 1,595 4 View
