Preparation of DNSA REAGENT?

More Abdulazeez Olamilekan Elemosho's questions See All

How can I put the number of client instead of simulation time?

I am working with opnet to ensure the scalability of Massively Multiplayer online games. Do you any way to put the number of client as an X-axis and the y-axis as a remote login or any others...

06 May 2015 9,417 2 View

Is there anyone have worked at Oversim and Omnet++?

I need to know how to start simulate at oversim and Omnet++

29 May 2014 6,567 0 View

Making cell suspension and staining

Hi, please could some one help me with the protocol for obtaining a cell suspension for hepatic and bone marrow cells? I am quite new to the field.

26 January 2010 1,742 2 View

How to Estimation of reducing sugar in maize flour?

I used the DNS method below for estimation of reducing sugar in maize flour but I'm having problem with step 3 because it took more than 13hrs to evaporate the ethanol. Is there any modified...

01 January 1970 1,583 4 View

Effect of March 2020 ASUU Strike action

What do you think will be the effect of the March 2020 strike and the way forward?

01 January 1970 6,717 4 View

What lubricant is suitable for the sintering of Hydroxyapatite in Mild Steel mold?

I'm having difficulty finding a suitable lubricant for the sintering of Hydroxyapatite

01 January 1970 6,911 3 View

What lubricant can be used for the easy removal of Compacted/ pressed Hyrdoxyapatite powder from Mild steel mold?

I'm having difficulty removing the the compacted block from the mold

01 January 1970 2,456 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How to inerpret whether PCR went OK?

Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...

14 February 2021 3,129 8 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View