The GelDoc developed faults for capturing the bands and would take weeks to get it fixed. Can I then preserve the gel for later viewing? Thanks
this question is aimed at unraveling the influence of political othering in electorates' choices of candidates in Nigeria's just concluded 2023 elections.
25 March 2023 7,634 0 View
I need an existing research work on the role of EFCC on assest recovery and return in Nigeria especially during Muhammadu Buhari led administration who is Keen toward fighting corruption.
27 June 2021 7,949 2 View
i have been trying to prepare 100 ml DNSA reagent according to this procedure. 1 g of DNSA, 0.4M sodium hydroxide and 30 g of sodium potassium tartrate, but the sodium potassium tartrate is not...
27 January 2020 8,307 2 View
Hello everyone, i am seriously in need of a step-by-step guidelines on how to use IntraCal 2 Analyzer for LCFAs measurement in a wastewater sample? or can anyone suggest another method(s) that...
29 October 2018 6,630 0 View
I am looking for: (1) Best and simple method of evaluating the size of the in-situ block before blasting and comparing this with mean fragment size obtained after blasting. (2) I want a...
12 September 2018 5,327 4 View
Please, I need a material on how about how technology transformed Artisan mining into advance/developed sector......Please, I will appreciate any response ASAP thanks in Advance!
11 February 2017 3,904 2 View
i have successfully extracted the Hydroxyapatite from Bovine bone. The new challenge is what casting methods can i employ to cast samples of Human tooth (Molar, Premolar, Incisors and Canine). How...
09 December 2016 2,834 3 View
Looking for comments on using semiotics to analyse YouTube text (specifically UGC videos).
06 December 2016 2,911 7 View
I am working with opnet to ensure the scalability of Massively Multiplayer online games. Do you any way to put the number of client as an X-axis and the y-axis as a remote login or any others...
06 May 2015 9,505 2 View
I am trying to carry out ICP-MS analysis
26 November 2014 9,648 9 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View