Preparation of 1% crystal violet for Candida biofilm total mass quantification

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What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Why oxidation with peracetic acid engender staining of oxytalan fibers with clastic tissue stains?

Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?

20 December 2020 5,878 1 View

Does UV spectrophotometry methods such as Bradford and Lowry methods applicable to estimation of total protein of food sample?

If yes, what preparations (or method) that we need to make for preparing food sample before using UV spectrophotometry methods? For my case, the purification of protein sample is not required as i...

21 August 2020 1,123 4 View

What causes the copy detection limitation in PCR?

Hi all, I wish to understand why doesn't all polymerase can detect a single copy of region of interest? While some polymerase is more sensitive (like dreamtaq), and some are less sensitive, what...

02 June 2020 4,397 21 View

How to Start My Research Regarding Microgrid ?

Hello there. I am new to research and my research area is about "Energy Management System in Microgrid". I had already studied some research papers to have a better understanding on that...

01 June 2020 1,046 6 View

I am reading journal paper about mangroves. And I would like to get some recommend journal related to dissolved oxygen influence on mangrove growth?

I am wondering, why my study result show that Kandelia candel and Avicennia alba species are most sensitive towards soil dissolved oxygen (DO) besides R. apiculata among the mangrove species in...

27 May 2020 1,366 3 View

Does anyone has experience with NEB "time-saver enzyme" overnight digestion?

Hi all, I used the Thermo-ANZA series for overnight digestion of plasmid backbone to ensure clean digestion without any problem. How about NEB? The website said all NEB "time-saver enzyme"...

24 March 2020 7,636 2 View

Difference between cell culture plates and flasks?

In the lab we always use culture plates (33, 60, 100, 150mm) for passing cells and conducting all sorts of experiments. I wonder what is the perks of using cell culture plates v.s. flasks? Should...

12 March 2020 1,361 2 View

Minitab Factorial Analysis - why do I get two different coefficients?

Hi everyone, I run factorial analysis on my data but obtained two different coefficients, coded & uncoded. Please could you advise which one should I use for results interpretation or have I...

09 March 2020 9,397 3 View

Why does my HPLC filter always generate air bubbles?

I have an old HPLC machine. Every time when I put my filter into 50%:50% water and acetonitrile mixture, my filter always generate bubbles. However, once I change the solvent to 100% acetonitrile,...

08 March 2020 5,587 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View