Preadipocytes SGBS cell line?

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What are novel cryptographic protocols that enhance email security?

what are novel cryptographic protocols that enhance email security?

13 June 2024 7,090 2 View

Hello, very good day everyone. I wanted to ask how many rpm to obtain the separation of a drug sample in wastewater with activated sludge?

I need to separate my drug from a sample with activated sludge and residual water

17 April 2024 9,601 2 View

How to get B.inp GGA for generate vdW pseudopotential of atom in SIESTA?

On the page I currently cannot download it, or if you can guide me to generate it.

21 February 2024 4,374 0 View

¿What is the affinity of serotonin (endogenous) for the 5-HT1 and 5HT2 receptors?

I need to know if there are measurements or studies on the affinity of serotonin (endogenous) for the 5-HT1 and 5-HT2 receptors

03 February 2024 5,878 3 View

Why MiFish Primers amplify when they're alone but not when they have an overhang sequence (Illumina adapter + tag)?

Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...

11 January 2024 5,109 2 View

In petrel, do you know why a well is correctly displayed in 2D window (checkshots are corrects) and not in a 3D windows ?

I have some issues with wells in Petrel: A well is at the right position in 2D interpretation window whereas it's not in a 3D window (see screenshots). I do not understand why. Any idea ? In 3D it...

08 December 2023 9,125 1 View

What are the specific challenges and methodological best practices when conducting a meta-analysis in studies related to cancer?

How can these methodological considerations impact the reliability of drawn conclusions and their relevance to cancer research and treatment? I am currently exploring the realm of meta-analyses...

20 November 2023 6,056 2 View

Do someone know the heat transfer coefficient of ice?

I just need the Hc

21 October 2023 5,162 3 View

ERA5's sensible heat radiation in W/m^2 is the same as mean sensible heat radiation?

If I convert J/m2 to W/m2 the reanalysis variable surface latent heat flux, will there be any difference with the variable mean surface latent heat flux? Or is it basically the same when you do...

15 September 2023 8,999 0 View

Predators of Thrips parvispinus?

Good evening: Does anyone know what are the natural predators of Thrips parvispinus? Thanks in advance Jesus

26 July 2023 4,659 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View