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Questions related from Asavari Ani Joshi
if I differentiate THP-1 cells using PMA and later in the experiment if I change the medium of THP-1 cells to xombination of MEM and RPMI, what is the possiblity that cells will dedifferentiate?
30 July 2025 1,682 1 View
Sodium salt of palmitic acid is conjugated with fatty acid free BSA (6:1 molar proportions). This is further diluted in 2% FBS containing culture medium to achieve desired concentrations (0.1-1.5...
25 February 2025 3,945 2 View
BSA conjugated FA treatment for cell culture is known. For triglycerols, most used method is dissolving TGs in ethanol and treating with it. But is it possible to get BSA complex of TGs or oil?...
23 August 2018 4,236 1 View
I want to know whether we can add oils (dissolved in DMSO) to animal cell lines instead of free fatty acids conjugated to BSA? whether cells will be able to take up and utilize these TAGs? I am...
22 August 2017 4,672 4 View
I will be performing ELISAs for cytokines but I have few doubts- 1. whether the ingredient and LPS should be added simultaneously? 2. While treatment, whether medium should be FBS free?
20 June 2017 5,395 3 View
I am working on hPL, after hPL treatment cell morphology is changed, cells look smaller and little stressed. There is fine deposition of granules on and around cells in hPL treated wells. This may...
24 November 2016 1,771 6 View
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22 March 2016 2,480 3 View
