Asavari Ani Joshi

6 Questions 10 Answers 0 Followers

Questions related from Asavari Ani Joshi

I want to co-culture HepG2 cells and PMA differentiated THP-1 cells. will differentiation be affected if I make changes in medium?

if I differentiate THP-1 cells using PMA and later in the experiment if I change the medium of THP-1 cells to xombination of MEM and RPMI, what is the possiblity that cells will dedifferentiate?

30 July 2025 1,662 1 View

Why sodium salt of palmitic acid is not inducing toxic effect in hepg2 cells?

Sodium salt of palmitic acid is conjugated with fatty acid free BSA (6:1 molar proportions). This is further diluted in 2% FBS containing culture medium to achieve desired concentrations (0.1-1.5...

25 February 2025 3,936 2 View

Is it possible to get triglyceride and albumin complex?

BSA conjugated FA treatment for cell culture is known. For triglycerols, most used method is dissolving TGs in ethanol and treating with it. But is it possible to get BSA complex of TGs or oil?...

23 August 2018 4,230 1 View

Can cells (in animal tissue culture) utilize triacylglycerols?

I want to know whether we can add oils (dissolved in DMSO) to animal cell lines instead of free fatty acids conjugated to BSA? whether cells will be able to take up and utilize these TAGs? I am...

22 August 2017 4,663 4 View

What is effect of hPL (human platelet lysate) on human adult dermal fibroblasts morphology?

I am working on hPL, after hPL treatment cell morphology is changed, cells look smaller and little stressed. There is fine deposition of granules on and around cells in hPL treated wells. This may...

24 November 2016 1,754 6 View

What are the markers for separating oral SCC cells (from patients) from normal cell?

22 March 2016 2,470 3 View