Composition of HEPES buffer solution?

Hi All, I want to make HEPES buffer solution and when I search for the composition I could find two different types of it. Can anyone help me which one is the correct method of HEPES...

08 September 2020 450 4 View

How to genotype Cre-mediated recombination events in ROSA26-pCAG-LSL KI mice?

Hello, I am using a mice line which is a cross product of Sun1-tagged mice (Jax #021039) with VIP-Cre mice (Jax # 010908). I'm trying to establish a PCR genotyping protocol to detect whether an...

20 April 2020 7,692 3 View

Why my HaCaT cells die after thawed?

I tried to thaw my HaCaT cells several times but 99% of them died. They were frozen with 10% DMSO + 10% FBS + 80% DMEM. I also tried 10% DMSO + 90% FBS then they died less but still only 10-20%...

18 October 2019 1,762 2 View

What could be the factors for unreasonably high heat capacity values from DSC measurements?

I have been running DSC for porous materials but the results I got were quite ridiculous. For the solid porous materials, I obtained heat capacity above that of water, which I cannot reason at...

20 September 2019 2,211 3 View

Do you have training courses to make models for my thesis on climate change and food security in Senegal?

Currently I am a student in Master II at the Faculty of Economics and Management of the University Cheikh Anta Diop Dakar.I finish this higher level with a dissertation whose theme is "the impacts...

01 June 2018 8,422 4 View

No title

Please click the link to take part in the short survey (less than 3 minutes) on the updated EMA Guideline on first-in-human clinical trials. Your data will be collected anonymously....

02 March 2018 7,673 0 View

Seaweed water content.

08 June 2017 5,789 3 View

How can I prepare an optimal nanoparticles suspension?

13 March 2017 2,927 3 View

Can I use a linearized plasmid that has been stored (in water) at minus 20 for two weeks?

Hello, I have a linearized plasmid (digested and cleaned by phenol/chloroform precipitation) that has been stored in water at minus 20 deg for two weeks. The plasmid is intended to be used as...

27 February 2017 5,388 4 View

Which are the best tests to assess behavioural consistency in two different populations of fish (grouped by sex, spawning success)?

I performed different coping style tests on two fish populations (grouped by sex and spawning success). Which tests are the most suitable to demonstrate the consistency of individuals (eg. I want...

30 June 2015 6,671 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View