can anyone please recomend me any primer
Dear Syeda, you can use T7 and T7-term primers for 5' and 3' sequencing, respectively. In the addgene website you can also find more information about your vector.
Vector link: https://www.addgene.org/26042/
I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.
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website,genome sequence
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in which i resuspend my pellet against which buffer i have to dialysis.
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TX6119- Fragments were cloned into the expressionvector pQE30 (Qiagen) and transformed into M15 (pREP4) cells(Qiagen).i want to make culture in LB broth to do miniprep i am confuse how much i add...
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Positive and negative control
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Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
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Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
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