website,genome sequence
https://www.ncbi.nlm.nih.gov/assembly/GCF_000174395.2/
REFERENCE 1 (bases 1 to 2698137) AUTHORS Qin,X., Galloway-Pena,J.R., Sillanpaa,J., Hyeob Roh,J., Nallapareddy,S.R., Chowdhury,S., Bourgogne,A., Choudhury,T., Munzy,D.M., Buhay,C.J., Ding,Y., Dugan-Rocha,S., Liu,W., Kovar,C., Sodergren,E., Highlander,S., Petrosino,J.F., Worley,K.C., Gibbs,R.A., Weinstock,G.M. and Murray,B.E. TITLE Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes JOURNAL BMC Microbiol. 12 (1), 135 (2012) PUBMED 22769602
thank you so much appreciated
I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.
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As PET duet vector is for cloning of two gene of interest;i want the protocol for it.
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is my plasmid is contaminated with ethanol;how to avoid this contaminated
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I run gel electrophoresis to confirm digestion of my plasmid however my samples are remaining at the start of the gel. I have remade wash buffers and carrier buffers but am still not seeing my...
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can anyone please recomend me any primer
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in which i resuspend my pellet against which buffer i have to dialysis.
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Tx6119( M15 containing fragment of SwpA26-226 from TX82 cloned into pQE30),when i run it as control without injecting IPTG i never saw any band on SDS-gel.
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Can anyone suggest why i am not getting any bands
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TX6119- Fragments were cloned into the expressionvector pQE30 (Qiagen) and transformed into M15 (pREP4) cells(Qiagen).i want to make culture in LB broth to do miniprep i am confuse how much i add...
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Positive and negative control
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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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Is this website real? https://isar.org.in/event/registration.php?id=2434532
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A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
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I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
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Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.
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TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
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Reference dose and Maximum acceptable concentrations HMs
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Molecular docking software/ websites?
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I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
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