Syeda Mahreen Hassan

12 Questions 16 Answers 0 Followers

Questions related from Syeda Mahreen Hassan

I want to do protein expression in E. faecalis JH22 by using anhydrotetracycline (ATc) to induce protein expression .Need protocol for expression?

I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.

07 July 2019 9,567 2 View

How to check the genome sequence of locus A of Enterococcus feacium TX16 species;any site for this ?

website,genome sequence

02 February 2019 7,218 2 View

I want to use PET-Duet vector but i donot protocol how to do restriction cutting with two enzymes ;if i want to insert two gene of interest?

As PET duet vector is for cloning of two gene of interest;i want the protocol for it.

07 July 2018 9,136 0 View

When i purified my digested plasmid from the agrose gel ;and try to run of the gel again it just float from the well.Is my plasmid is contaminated ?

is my plasmid is contaminated with ethanol;how to avoid this contaminated

01 January 2018 4,344 0 View

Why are my gel electrophroesis samples are not moving?

I run gel electrophoresis to confirm digestion of my plasmid however my samples are remaining at the start of the gel. I have remade wash buffers and carrier buffers but am still not seeing my...

01 January 2018 6,293 0 View

POPINF plasmid i want to send for sequencing i donot what type of primer i have to use company have standard primers?

can anyone please recomend me any primer

11 November 2017 606 1 View

Ammonium sulphate precipitation method for protein , after centrifugation when i got the pellet,in which buffer dialysis of pellet has done to NMR?

in which i resuspend my pellet against which buffer i have to dialysis.

11 November 2017 5,630 2 View

I want to express my TX6119 strain ;i run it as control without injecting IPTG i saw no band?

Tx6119( M15 containing fragment of SwpA26-226 from TX82 cloned into pQE30),when i run it as control without injecting IPTG i never saw any band on SDS-gel.

10 October 2017 3,242 4 View

I am using Sybre Safe to stain the gel electropheresis;i 5microlitre in each welll but donot got any band, i just saw ladder band ,i am adding 6x dy?

Can anyone suggest why i am not getting any bands

10 October 2017 5,733 3 View

How much i add amipicillin and kanamycin into pQE30 (Qiagen) contains cloned fragment and transformed into M15 ( (pREP4) cells to do miniprep?

TX6119- Fragments were cloned into the expressionvector pQE30 (Qiagen) and transformed into M15 (pREP4) cells(Qiagen).i want to make culture in LB broth to do miniprep i am confuse how much i add...

08 August 2017 378 4 View

What type of positive and negative control;can be use for Histag antibody in Western blot?

Positive and negative control

06 June 2017 5,981 3 View

Growing the bacteria(LB broth) for protein expression: the optical density(1.2) , is this good for protein expression(before IPTG).

growing the bacteria(LB broth) for protein expression: the optical density(1.2) , is this good for protein expression(before IPTG).

04 April 2017 3,212 13 View