Tx6119( M15 containing fragment of SwpA26-226 from TX82 cloned into pQE30),when i run it as control without injecting IPTG i never saw any band on SDS-gel.
IPTG is an analog of allolactose and either lactose or IPTG (preferably IPTG because it is not metabolized) is required to induce lac operator and initiate transcription. pQE30 vector contains lac operator, therefore in absence of IPTG your cloned fragment will not be transcribed and translated. Hence, no band of SDS-gel.
If your aim is to observe the effects of IPTG in expression, I would say your control is working just fine. For other purposes, I daresay you might have to redefine your control.
Well, to get a band for SwpA26-226, you will still have to add IPTG to the media, same as the different protein you want to observe. I don't see why you wouldn't add IPTG to the control.
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