PMA induced in THP-1 cell culture

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Alexander Gluschko

Hi,

the THP-1 cells can migrate after PMA differentiation into macrophage-like cells. I think without PMA differentiation your THP-1 cells won't become adherent and it will be difficult to analyze cell migration.

Michael Förster

Dear Melonney Patrick

Please see attached publication for a side by side comparison of THP-1 and PBMC derived macrophages in a standard chemotaxis assay using HUVECs.

https://www.frontiersin.org/articles/10.3389/fphar.2018.00071/full

I hope that helps when designing your experimental protocol.

All the best & kind regards,

Michael