Jibin, 

here only 3 questions for honest reasons:

1) what is your (scientific, theoretical, practical) background and experience ( novice, newcomer, at least to some degree an expert?) with respect to specimen processing of "microorganisms" (? too it would be wise to tell the type of MO as well as the NP used or intended to be used to the !)

2) you wish to do transmission electron microscope (as I understood from your question). What is the brand (and capabilities) of the (T-)EM you might use after a preparation / processing of specimens?

3) Have you tried any time for this your "project" to find at least SOME scientific literature / articles / or general book sources dealing with the theme (e. g. Toxic effects of Nanoparticles leading to destruction of ??microorganisms??) ? I guess you are not talking about NP-induced destruction of cell(s) ending up with their (cell) death.

Would be nice to get answers...   Regards, Wolfgang

Jibin, 

here only 3 questions for honest reasons:

1) what is your (scientific, theoretical, practical) background and experience ( novice, newcomer, at least to some degree an expert?) with respect to specimen processing of "microorganisms" (? too it would be wise to tell the type of MO as well as the NP used or intended to be used to the !)

2) you wish to do transmission electron microscope (as I understood from your question). What is the brand (and capabilities) of the (T-)EM you might use after a preparation / processing of specimens?

3) Have you tried any time for this your "project" to find at least SOME scientific literature / articles / or general book sources dealing with the theme (e. g. Toxic effects of Nanoparticles leading to destruction of ??microorganisms??) ? I guess you are not talking about NP-induced destruction of cell(s) ending up with their (cell) death.

Would be nice to get answers...   Regards, Wolfgang

thank u sir for your response

1) i am doing msc microbiology in VIT university.we synthesised  silver nanoparticle with the help of micoorganism ,isolated from soil. we want to find the disinfectant capability of silvernano particle which we synthesised.we r planning to test with Ecoli,Pseudomonas,Klebsiella  & staph

and i am new to sophisticated technique TEM

2)Yes sir,sorry i don't have any idea about brand or capability of the instrument.if you could  recomment that it will be help full.

3)This is the first time I am doing it.Have many article related to this eg:Comparison of bactericidal activities of silver nanoparticles with common chemical disinfectants

Karthik Chamakuraa,d, Rafael Perez-Ballesteroa, Zhiping Luob, Sajid Bashirc, Jingbo Liud,e

Hi Jibin,

What you're asking for will require a bit of work. Is there anyone at your uni that is an expert at biological sample preparation. For us to talk you through it would be hard -  there are books for sample preparation of biological samples. A lot of small steps during sample preparation, can ruin your samples if you are not careful.

From the paper that you provided, the technique they use would require someone with a bit of experience. You will definitely need someone who knows what they are doing.

I suggest that you may want to ask your professor if they know anyone first.

Good luck

Ok, back from holidays....

Jibin, usually (IMHO) nobody would be able to give you any founded and sound answer to your question as you stated initially. The presentation and information on important cornerstones of your project are the / a basis for a scientifically correct answer.  

From your reply (2) to my initial 3 questions (1) I deduce the following:

i) You would like to perform Electron Microscopy but don't know anything about a / the certain or definitive (EM-)instrument you can use (at least in some vicinity to your facility, or even if there exists an EM-facility proper at your VIT-University. In the “Departments” Section (on RG) of the VIT-University I could not find any hint on an Institute of / for , or or some  other department eventually dealing and owning Electron Microscopes. I am guessing also about other “modern, state-of the art”-microscopy techniques and microscopes used in any of the VIT-University departments.

So IMHO it would first be necessary to find out whether there is a department providing “microscopy techniques” in general. Second would be to locate (if available) a department / institute having at least ONE TEM=transmission EM (SEM=Scanning Electron Microscopy  perhaps for beginning the study making no sense). You can start with classical TEM (meaning that also an TEM is sufficient for producing any valuable result regarding ultrastructural alterations and antibacterial effects of your silver nanoparticles in your bacterial cultures, at least if it is operated appropriately).

ii) If you can locate such an instrument (either at your own University or another in your area) contact that facility (find out the head of the institution and talk to the person personally, telling what your project is).

iii) This person would be well informed about the technical as well as methodological possibilities the facility can provide. Also they can tell you which knowledge YOU must have to get into the “scientific business” which not necessarily will be “only scientific” but also a lot of bench-working with your own hands on.

iv) Once you have completed such a you can inform us again at RG about the outcome and conditions you will be allowed / will be able to do your research.

v) In parallel you first should think also over a “light microscopic” approach, i. e. find out and record results of an experiment using your  < synthesised silver nanoparticle with the help of micoorganism, isolated from soil.>>, the effects of which should be also present and demonstrable by either plain / classical LM (LM-BF=bright field) as well as more sophisticated “new” LM-(and the like) techniques (including e. g. Fluorescence Microscopy).

vi) You have to learn and inform yourself about “specimen preparation of biological material/samples for electron microscopy” (handbooks and books dedicated to processing biomaterials including bacteria for microscopy) and naturally know about / and have at hand then all those special chemicals (or physical implementations like cryo-specimen preparation instruments)  and perhaps also sophisticated/specific apparatuses an EM-Lab must have (including specified working areas and e. g. fume cupboards).

vii) Last but not least, if you are “novice” with the intended techniques (either LM or also EM-TEM) you have to develop also a methodological strategy how to deal (personally) with all those dangerous/hazardous bacterial samples (personal safety equipment and protection).

Best regards, Wolfgang

thank u sir for your valuable response @Wolfgang

Dear Jibin, you're welcome.