13 September 2013 1 5K Report

My protein has carbohydrate binding modules. Suggestions are required to study if the protein binds/interacts with plant carbohydrates.

More Madhurima Kahali's questions See All
Can I use 1 M tris HCl buffer instead of 1.5 M in SDS-PAGE resolving gel?

I don't have 1.5M tris HCl buffer for the resolving gel but I have 1 M tris HCl buffer with pH 8.8 will that work for SDS PAGE? do I need to change the amount added to the mix?

11 June 2024 5,753 3 View

Hi, it is my request to all, can any body provide me with CD45 mouse isoform primer sequences like RA,RB,RC, RAB,RBC, RABC and R0?

I am working with mouse bone marrow. Need CD45, six isoform primer sequences (mouse).These isoforms are generated through alternative splicing of exon 4,5 and 6. NCBI Reference Sequence:...

13 February 2024 7,041 0 View

Characterisation test for proteins extracted in an acedic media?

i am working with lyophilised porcine skin. what tests can be carried out on the extracted sample to determine what proteins/ components are present?

08 February 2024 9,129 0 View

Please share lowry assay protocol for protein estimation?

please share an easy-to-follow protocol of lowry assay, along with the method to prepare BSA standard graph in the range of 5ug/ ml to 100ug/ml

16 August 2023 8,572 0 View

Method for protein extraction from porcine skin?

hi, I am working on extracting proteins from pig skin. Initially, I used plain 0.5 M acetic acid solution for extracting proteins and used the solution as such post-centrifugation. but the protein...

31 July 2023 5,737 3 View

How to determine the end point while defatting pig skin using surfactant?

I am working with protein extraction using porcine skin. prior to protein extraction I need to de-fat the tissue. I am using triton x to de-fat the tissue... how to determine if the defatting is...

19 July 2023 9,930 4 View

We have a Beckman DU 530 UV spectrophotometer in lab, I want to get some suggestions about which would be the best way to calibrate the instrument??

Please, share any links or papers that can help me know more about the ways to calibrate the instrument and how regularly the calibration must be done. Thank You for the help.

20 August 2021 4,839 3 View

Can study based on semistructured interview be done on a construct with pre existing scale?

I am planning to do a study on emotional intelligence of managers...

24 July 2021 9,430 7 View

What are the mental health insurance policies in different countries?

Post Covid 19 the scenario in India has changed and some of the private insurance companies are providing coverages for mental disorders. So My question is what mental disorders are covered in...

04 January 2021 6,709 10 View

I am trying to do retroviral transduction using platE cells but its not giving good efficiency.Can somebody suggest ways to increase efficiency ?

I use Fugene-3ul/well, pclEco- 0.8ng and polybrene 2ul/ml from 5mg/ml stock. I usually spinfect T cells that I have activated a day before with anti-CD28 and anti-CD3. I use MigR2 plasmid with my...

08 December 2019 3,791 3 View

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