Madhurima Kahali

5 Questions 12 Answers 0 Followers

Questions related from Madhurima Kahali

After selfing of T0 transgenic plants, will all the subsequent T1 plants that I get will have the same T-DNA integration pattern?

does selfing T0 transgenic plants, give genotypically identical T1 progeny lines? Will these have the same T-DNA integration (can I observe that in Southern hybridization experiments). will all...

08 December 2014 9,684 7 View

What is the best internal control for Nicotiana tabacum?

I'm doing RT-PCRs. Which gene is considered a good internal control for tobacco?

18 September 2014 9,733 5 View

Can docking be tried out with polysaccharides?

My protein of interest has a carbohydrate binding module-like domain, can I check if it binds to cellulose/pectin/xylans?

23 October 2013 10,077 2 View

How do you check for any sort of post-transcriptional/translational silencing in genes?

My transgenic is positive for DNA (in PCR) and transcript (in RT-PCR) of my transgene, but when I did an ELISA there was no protein expression observed. There must be some sort of silencing...

10 September 2013 3,780 13 View

How to check if my protein of interest interacts with plant actin?

What would be the best way to study the interaction? Purifying actin and doing on gel binding assays or tagging actin and checking for interaction?

03 September 2013 8,173 8 View