please share an easy-to-follow protocol of lowry assay, along with the method to prepare BSA standard graph in the range of 5ug/ ml to 100ug/ml
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Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
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What means gamma in old chemistry papers like Lowry method? I have a suspicion it was the concentration unit in micro something. I am right? Why they used it? Typographical problems at the time?
22 January 2024 9,232 2 View
We need to accurately measure the protein/peptide concentration in a sample that is at least 80% DNA (primarily ssDNA less than 80 bases long with some dsDNA also). A majority of the methods I've...
24 September 2023 9,156 3 View
I am trying to check a purified recombinant protein on SDS PAGE coomasie Blue and silver staining. Before SDS PAGE, I have checked the total protein concentration using lowry and it shows good...
03 July 2023 8,988 4 View
Iam working on seaweed protein. After extraction of protein, i have precipitated with ammonium sulphate and freeze dried the sample after dialysis. In kjeldahl iam getting good percentage of...
18 April 2023 7,611 0 View
Hi all, I am looking into purchasing the Thermo Scientific Pierce Modified Lowry Protein Assay Kit (Catalog number: 23240) to quantify total protein amount in mammalian cell lysates. I lyse my...
14 April 2023 7,998 7 View
Hello to everyone. My work focus on studying PTMs and I have successfully phosphorylate my protein in vitro (Checking the reaction products by SDS-Page, all of my protein should be...
29 March 2023 1,640 3 View
Special greeting. I have a protein extraction solution containing 1% SDS, 300 mM B-mercaptoethanol, 100 mM Tris and 8 M urea, 10 mM EGTA. Is there a method that allows me to measure protein...
28 March 2023 2,339 4 View
1. Basic concept of Crude protein and soluble protein? 2. Is lowry et al 1951 method used for estimation of Total soluble protein content in mungbean?? 3. If method has been changed then value...
04 March 2023 4,258 0 View
I used Lowry Technic. I prepared standards from BSA for make up standard curve. And then, ı measured my samples proteins. How can ı find protein contents in my samples?
17 January 2023 2,199 2 View
I want to measure protein content in my plants. So, ı will use Lowry method. How can ı prepare extraction buffer for plant materials?
18 December 2022 8,462 0 View